摘要
以神经微丝及红细胞膜为模型，选用ＳＤ雄性大鼠整体染毒２个月。从脊髓分离神经微丝，超速离心法分离红细胞膜，用变性聚丙烯酰胺电泳技术及薄层扫描技术观察ＣＳ２所致该两种结构的改变。结果表明：染毒大鼠神经微丝亚单位含量减少；红细胞膜电泳图谱薄层扫描分析有异常区带形成，推测该异常区带可能为红细胞膜收缩蛋白α、β亚单位发生共价交联所形成的异质二聚体，且其含量与ＣＳ２接触浓度有关。
In this study,the degradation of neurofilament protein and modification on erythrocyte spectrin was demonstrated in CS 2 treated rats at an exposure level of 1 200,2 400mg/m 3 by inhalation for two months.Spinal cords were removed from both CS 2 treated and non treated rats and NF riched cytoskeletal protein from spinal cords was prepared.A singnificant decrease was found in two subunit(200 and 70KDa)of neurofilament by quantitative assessment of cytoskeletal protein using Dual wavelength TLC scanner.The erythrocytic membrane was separated by ultra centri fugation.Covalent crosslinking of erythrocyte spectin was determined by SDS PAGE and TLC scanner.An abnormal high molecular weight band was found in CS 2 treated animals and a dose effect relationship was observed between the content of abnormal structure and CS 2 exposure level.It is suggested that the covalent cross linking of proteins has been considered as a potential mechanism for toxicity of carbon disulfide.
出处
《中国工业医学杂志》
CAS
1998年第5期267-270,共4页
Chinese Journal of Industrial Medicine
关键词
二硫化碳
神经微丝
红细胞膜
共价交联
Carbon disulfide, Neurofilament(NF), Erythrocyte spectrin, Cross linking
