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4-HPR促髓母细胞瘤细胞凋亡的机制

N-4-Hydroxyphenyl Retinoid-Induced Apoptosis is Associated with Down-Regulation of Bcl-xL in Human Medulloblastoma
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摘要 目的探讨4-羟苯维胺酯对髓母细胞瘤细胞株的促凋亡的效应,及其对抗凋亡相关基因BclxL表达的影响。方法将2.5μM的4-羟苯维胺酯作用于髓母细胞瘤D283MED细胞株,用MTT实验,克隆形成实验检测4-羟苯维胺酯对细胞增殖的抑制作用,TUNEL法检测瘤细胞的凋亡率。采用RT-PCR及Western blot方法检测Bcl-xL的mRNA和蛋白的表达。结果4-羟苯维胺酯可显著抑制D283MED瘤细胞的增殖,明显促进瘤细胞凋亡(P<0.05);RT-PCR及Western blot结果显示Bcl-xL的mRNA和蛋白均明显减少。结论4-羟苯维胺酯通过下调Bcl-xL基因及其蛋白的表达,从而抑制髓母细胞瘤D283细胞的增殖,促进瘤细胞凋亡。 Objective To investigate the effects of N-4-hydroxyphenyl retinoid（4-HPR）-induced apoptosis and the expression of Bcl-xL in human medulloblastoma D283MED cell line. Methods Human medullo- blastoma D283MED cell line was cultured in the medium containing 4-HPR（ 2. 5 μM ） . The ability of ATRA to inhibit glioma cells proliferation was examined by cell viability analysis （MTT assay） and plate colony formation assay. The apoptosis was detected by TUNEL assay. The expression of Bcl-xL mRNA and protein in D283MED cells was detected by RT-PCR and Western blot respectively. Results 4-HPR inhibited cell proliferation and caused apoptosis in D283MED cell line. 4-HPR down-regulated the expression levels of Bcl-xL mRNA and protein. Conclusion 4-HPR might inhibit the proliferation of D283MED cells by down-regulating the expression of Bcl-xL.
作者 王永谦 陆建荣
机构地区 上海中医药大学附属龙华医院神经外科 陕西省肿瘤医院病理科
出处 《肿瘤防治研究》 CAS CSCD 北大核心 2010年第1期5-8,共4页 Cancer Research on Prevention and Treatment
关键词 4-羟苯维胺酯 细胞增殖 凋亡 BCL-XL基因 人髓母细胞瘤D283细胞系 4-HPR Cell proliferation Apoptosis Bcl-xL Human medulloblastoma cell line
分类号 R739.41 [医药卫生—肿瘤]
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参考文献8

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二级参考文献49

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肿瘤防治研究
2010年 第1期

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