摘要
目的:建立与改进纯度较高的适于实验研究的人绒毛膜滋养层细胞。方法:采用胰蛋白酶消化法消化人正常妊娠6～8周胎盘组织,以35%、45%2个Percoll密度梯度进行分离纯化,并用免疫组化及透射电镜等技术对其生物学特性、细胞内部结构进行观察。结果:胎盘组织中滋养层细胞角蛋白染色阳性,血管内皮细胞及基质成分波形蛋白染色阳性,经该法分离纯化的细胞角蛋白染色阳性者(滋养层细胞)占90%以上,透射电镜观察示所获细胞有典型滋养层细胞结构。结论:该法简便易行,可获得合乎实验要求的人滋养层细胞,可供后续实验研究。
Objective：To prepare highly purified functional human trophoblast cells from human placentae.Methods：A trypsin-DNase digestion method was used to isolate the trophoblast cells.The cell population was identified by immunocytochemical staining method.The structural features were observed by transmission electron microscopy. Results：Light microscopic examination revealed patchy spreading and irregular polygonal cells with big and oviform nuclear and transparent cytoplasm.Cytokerat in positive cells were more than 90%.Transmission electron microscopy revealed that the cells had fine structural features typical of trophoblasts.Conclusion：Trophoblasts may be obtained with this method and could offer a model to for ongoing experiment.
出处
《生物技术通讯》
CAS
2010年第1期58-61,共4页
Letters in Biotechnology
基金
国家自然科学基金(30771891)
关键词
滋养层细胞
培养
体外
免疫组化
透射电镜
trophoblast cells culture in vitro immunocytochemical staining transmission electron microscopy
