摘要
目的进一步探讨二硫化碳（ＣＳ２）对男性生殖系统的毒性。方法用生物素标记的α－卫星Ｘ染色体特异ＤＮＡ探针与接触ＣＳ２工人精子ＤＮＡ进行荧光原位杂交（ＦＩＳＨ），测定Ｘ染色体非整倍体率。结果厂空气中ＣＳ２平均浓度高于国家最高容许浓度１～３倍，ＣＳ２作业工人血睾酮（Ｔ）含量降低，间质细胞刺激素（ＦＳＨ）及黄体生成素（ＬＨ）含量升高；１１位工人总共计数６０３４４条Ｘ精子，Ｘ精子双体率为０．０８２％±０．０２２％，高于正常健康人的０．０６３％±０．０１３％，差异有显著性（Ｐ＜０．０５）。结论接触ＣＳ２男工妻子流产率增加可能与男工精子染色体非整倍体率增加有关。
Objective To study futher the toxic effect of CS 2 on male reproductive system. Methods The frequencies of human sperm aneuploidy for chromosome X were detected by using fluorescence in situ hybridization(FISH) with α satellite X chromosome specific DNA probe. Results The air concentration of CS 2 in workshop and the whole factory was 1￣3 fold higher than that maximum allowable air concentration of our country. The sex hormones level in blood of the workers showed that there were marked decrease in the level of testosterone and significant increase in the level of follicle stimulating hormone(FSH) and luteinizing hormone(LH). A total number of 60 344 X sperm nuclei were scored in 11 workers exposed to CS 2. The mean frequency of disomic sperm for chromosome X was 0.082%±0.022%. A statistic difference was presented when this value was compared with that control value 0.063%±0.013%, which was measured in 10 normal healthy men. Conclusion It seems that the increased frequency of sperm aneuploidy in CS 2 exposed workers may be a cause of higher abortion rate in wives of CS 2 exposed workers.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
1998年第4期197-200,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金
中华医学基金
关键词
荧光原位杂交
二硫化碳
精子
染色体
双体率
Fluorescence in situ hybridization(FISH) Workers exposed to carbon disulfide Frequency of disomic sperm for X chromosome
