摘要
【目的】了解芽变植株的植物学特性及遗传背景。【方法】以普通无核白做对照,对其10个芽变株系的生物学和植物学特性进行观测,并对其幼叶进行RAPD分析。【结果】芽变株系在植物学特征上存在不同程度的变异,主要体现在果实性状上。【结论】初步发现1株4倍体芽变。通过RAPD分析,采用470个10碱基随机引物进行RAPD多态性检测,共筛选出能产生多态性标记的RAPD引物3个。
[ Objective and Memod] The study was carried out to observe the botanical and biological characters of thomson seedless bud variation and analyze young leaves by RAPD, using thomson seedlings as the control. [Result]There were different variations in bud variation of botanical characters. It was found that there was a bud variation with tetraploid in the test. [Conclusion]Through RAPD analysis, by using 470 random 10- alkali primers, RAPD polymorphism were detected from 30 samples. The polymorphism information could be provided with 3 random primers.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2010年第1期110-114,共5页
Xinjiang Agricultural Sciences
基金
国家科技支撑计划(2007BAD36B08)
