摘要
开展了郁金药材中姜黄素类成分的含量测定的研究，确立了姜黄素的优化提取条件，建立了姜黄素的高效液相色谱含量测定方法。通过筛选，发现以60％乙醇回流，15倍药材溶剂量，提取时间1h，提取次数1次对姜黄素的提取较完全。姜黄素在0．0265～0．2648μg范围内线性关系良好。精密度、稳定性和重复性的RSD均小于2％，平均加标回收率为99。73％，RSD为1．41％。
A method determine the content of Curcuma louga L. by HPLC. Was studied the most proper extraction condition for Curumin in Curcuma louga L. The experiment results indicate that the best extraction method is to keep the distilling content of 60% ethanol 15 times to Curcuma louga L. and to keep distilling time within 1 h every time. After been distilled for 1 time, it is extracted totally. Curumin were on the linear of 0. 026 5 - 0. 264 8 μg. The RSD of recurring, stabilization and precision were less than 2%. The recovery was 99.73% and it is RSD was 1.41%.
出处
《广东微量元素科学》
CAS
2009年第12期41-44,共4页
Trace Elements Science
