摘要
目的：同时测定不同采收期江香薷药材中木犀草素、黄芩素-7-甲醚的含量。方法：采用Angilent Zorbax，Extend C-18 （4．6mm×250mm，5μm）色谱柱；以甲醇-0．4％磷酸水溶液（体积比为50：50）为流动相进行等度洗脱，柱温：35℃；流速：1．0mL／min；榆测波长：350nm。结果：木犀草素、黄芩素-7-甲醚分别在0．04～0．61μg、0．20～3．01μg范围内线性关系良好，相关系数均为0．9999。药材提取方法的平均回收率分别为99．54％、98．60％，精密度、稳定性及重复性均良好。不同采收期江香薷药材中木犀草素和黄芩素-7-甲醚含量有较大差异，以8月份药材中木犀草素和黄芩素-7-甲醚含量最高，分别为264．8μg／g、2746．3μg／g。结论：所建立的方法灵敏快速，操作简便，重现性好，可用于江香薷药材的质量控制。
AIM： To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times. METHODS： The Angilent Zorbax,Extend C-18 column（4.6 mm × 250 mm, 5 μm） was used with methanol-water （containing 0.4% phosphonic acid）（50： 50） as mobile phase. The flow rate was 1.0 mL/min. The detection wavelength was set at 350 nm. RESULTS ： The method had the good linear relationship within the range of 0.0d-0.61 μg for luteolin and within the range of 0.20-3.01μg for negletein, respectively, with the correlation coefficient of 0. 999 9. The average recoveries of the two flavones were 97.32%, 98.60%, respectively. The contents of luteolin and negletein in Mosla chinensis Maxium. varied in different picking times, which reached the its maximum at florescence （264. 8 μg/g, 2 746. 3 μg/g ） in August, respectively. CONCLUSION： The method is fast, rapid, convenient, accurate and can be used for quality control of Mosla chinensis Maxium.
出处
《中成药》
CAS
CSCD
北大核心
2010年第1期88-91,共4页
Chinese Traditional Patent Medicine
基金
国家"十一五"科技支撑计划项目(2006BAI06A01-01)
