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乳铁蛋白对口腔癌细胞血管内皮生长因子和碱性成纤维细胞生长因子表达的影响 被引量：2

Effects of lactoferrin on vascular endothelial growth factor and basic fibroblast growth factor expression in human Tca8113 cell line
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摘要 目的观察乳铁蛋白对口腔癌细胞株Tca8113细胞中血管内皮生长因子（vascular endothelial growth factor，VEGF）和碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）表达的影响。方法实验分0．006、0．013、0．025、0．050g／L乳铁蛋白实验组和空白对照组。反转录聚合酶链反应（RT—PCR）和蛋白质印迹法分别检测VEGF、bFGFmRNA和蛋白表达的改变。结果0．050g／L乳铁蛋白实验组VEGFmRNA的表达（0．31±0．08）和蛋白的表达（0．68±0．11）均显著低于空白对照组[分别为（1．07±0．13）、（0．97±0．07）]，P〈0．05；0．050g／L乳铁蛋白实验组bFGFmRNA的表达（0．27±0．10）和蛋白的表达（0．68±0．07）均显著低于空白对照组[分别为（0．91±0．12）、（1．01±0．09）]，P〈0．05。结论乳铁蛋白能抑制口腔癌细胞株Tca8113细胞中VEGF、bFGF的转录和表达，抑制肿瘤的血管发生可能是乳铁蛋白抗口腔癌的机制之一。 Objective To investigate the effect of lactoferrin on vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF）expression. Methods Lactoferrin at concentration of 0. 006,0. 013,0. 025,0. 050 g/L and blank control groups were included in this study. The gene and protein expression of VEGF, bFGF were examined by RT-PCR and Western blotting. Results The RT-PCR and Western blotting assay showed that VEGF mRNA （0. 31 ± 0. 08 ） and protein （0. 68 ± 0. 11 ）in lactoferrin （0. 050 g/L） group were significantly lower than in the control group （ P 〈 0.05 ）, and the bFGFmRNA （0. 27 ± 0. 10） and protein （0. 68 ± 0. 07 ） in lactoferrin （0. 050 g/L） group were also significantly lower than in the control group （ P 〈 0. 05 ）. Conclusions Lactoferrin could inhibit the expression of VEGF, bFGFmRNA and protein in Tca8113 cells. This effect might be one of the mechanisms for anticancer function of lactoferrin.
作者 王雷 魏秀峰 孙宏晨
机构地区 吉林大学口腔医学院口腔黏膜病科 吉林大学口腔医学院口腔病理科
出处 《中华口腔医学杂志》 CAS CSCD 北大核心 2010年第1期28-30,共3页 Chinese Journal of Stomatology
基金 吉林省科技发展计划（200705346）
关键词 乳铁蛋白 口腔肿瘤 血管内皮生长因子类 Lactoferrin Mouth neoplasms Vascular endothelial growth factors
分类号 R739.8 [医药卫生—肿瘤]
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参考文献9

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二级参考文献23

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共引文献5

同被引文献24

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中华口腔医学杂志
2010年 第1期

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