摘要
目的:研究转录因子c-Jun及下游蛋白MMP-9在胃癌组织中的表达状况,分析其与临床病理特征和生存之间的关系。方法:应用免疫组化SP法,采用组织芯片技术,分析189例胃癌组织、54例癌旁组织、41例淋巴结转移灶及32例正常对照组织中c-Jun和MMP-9蛋白表达。结果:胃癌中c-Jun和MMP-9蛋白的阳性表达率分别为73.0%和78.3%。c-Jun蛋白的表达与分化程度有关(P<0.05);MMP-9的表达与浸润深度、淋巴结转移、分化程度及Lauren分型有关(P<0.05)。胃癌中c-Jun和MMP-9蛋白表达水平呈正相关(P<0.05)。41例淋巴结转移灶和相对应的41例胃癌组织各因子表达比较发现,MMP-9阳性表达率有显著性差异(P<0.05),c-Jun阳性表达率无显著性差异(P>0.05)。单因素生存分析显示c-Jun和MMP-9阳性组对胃癌患者生存期的影响有统计学意义(P<0.05),COX多元回归分析显示c-Jun和MMP-9的异常表达均不可作为影响胃癌预后的独立因素(P>0.05)。结论:c-Jun和MMP-9在胃癌组织中均有高表达。c-Jun阳性表达与胃癌的组织学分级密切相关,提示其异常表达可能是胃癌发生过程中的一个早期分子事件。MMP-9阳性表达与肿瘤的浸润、转移密切相关,可作为判断胃癌恶性行为的重要生物学标记物。c-Jun和MMP-9蛋白表达水平呈正相关。c-Jun和MMP-9蛋白阳性表达对于胃癌患者是一个不利的预后因素,尚不能作为一个独立的预后指标。
Objective： To investigate the expression of c-Jun and MMP-9 in gastric cancer tissues, para-cancerous tissues and metastastic lymph nodes, and to explore its role and significance for the clinicopathology and prognosis. Methods： Immunohistochemistry was employed to detect the expression of c-Jun and MMP-9 in tissue microarrays containing gastric normal mucosa （n=32）, para-cancerous tissues （n=54）, metastastic lymph nodes （n=41）, and gastric cancer tissues （n=189）. Results： The positive rates for c-Jun and MMP-9 expression in gastric cancer were 73.0% and 78.3%, respectively. The positive rates of c-Jun protein was significantly associated with the degree of differentiation （P〈0.05）, but was not associated with the depth of invasion, lymph node metastasis, Lauren type, sex, age or size of tumor （P〉0.05）. The positive rates of MMP-9 was significantly associated with the depth of invasion, lymph node metastasis, Lauren type and degree of differentiation （P〈0.05）, but was not associated with sex, age or size of tumor （P〉0.05）. The positive rates of MMP-9 expression in the 41 gastric cancer tissue samples and 41 metastastic lymph node tissue samples were significantly different （P〈0.05）. In metastastic lymph nodes, the positive rate of MMP-9 expression was higher. Kaplan-Meier survival analysis showed that the survival rate of patients with negative c-Jun and MMP-9 expression was higher than that of patients with positive c-Jun and MMP-9 expression （P〈0.05）. COX regression analysis showed that c-Jun and MMP-9 expression was not independent prognostic factor for gastric cancer. Conclusion： The expression of c-Jun is positively associated with the degree of differentiation. The increased c-Jun expression maybe an early indicator of gastric cancer. The high expression of MMP-9 may involve the occurrence, development, invasion, and metastasis of gastric cancer. C-Jun and MMP-9 are useful markers for predicting the outcome of gastric cancer, but they are not independent prognostic factors.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2010年第1期19-22,28,共5页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金资助(编号:30872478)
