摘要
用体外培养法研究ＧＨＭ１０对Ｌ１２１０细胞生长的抑制作用和作用机制。结果表明，Ｌ１２１０细胞在用ＧＨＭ１０处理１ｈ，２４ｈ和７ｄ后，ＩＣ５０依次为６８５，３３２和１５９μｇ·ｍｌ－１，提示ＧＨＭ１０有非时相特异性细胞毒药物的特性。在用ＧＨＭ１０１～２μｇ·ｍｌ－１作用２４ｈ后，Ｌ１２１０细胞的增长率和有丝分裂指数下降，细胞核形态发生变化，但活细胞率仍保持在９６％以上，提示ＧＨＭ１０主要抑制细胞的增殖。用流式细胞计对Ｌ１２１０细胞进行细胞周期动力学的分析表明，ＧＨＭ１０可在一定程度上阻断Ｇ１期细胞向Ｓ期移行。
Howiinol(GHM10) is a new compound isolated from Goniothalamus howii. Previous studies showed that GHM10 significantly inhibited the growth of cancer cells both in vitro and in vivo. In the present study, the characteristics of the inhibitory effect of GHM10 on L1210 cells was investigated. The results showed that the IC50 of L1210 cells treated with GHM10 for 1 h, 24 h, and 7 d was 685, 332 and 159 μg·ml-1, respectively. This outcome is in compliance with the hypothesis that GHM10 is a cell cycle nonspecific cytotoxic drug. When L1210 cells were treated with 1～2 μg·ml-1 of GHM10 for 24 h, the growth rate and mitotic index were decreased and the morphology of cell nuclei changed, but the cell viability remained at the level of 96% or more, indicating that GHM10 mainly inhibited cell proliferation. The cell cycle kinetics of L1210 cells were studied by using a flow cytometer. The results showed that the transition of cells from G1 phase to S phase was blocked to certain extent by the treatment of GHM10 4 μg·ml-1 for 12 h. The fluidity of cell membrane was also increased when the L1210 cells were exposed to 2～3 μg·ml-1 of GHM10 for 24 h.
出处
《药学学报》
CAS
CSCD
北大核心
1998年第8期566-570,共5页
Acta Pharmaceutica Sinica
