摘要
通过荧光光谱法和紫外吸收光谱法研究了超氧化物歧化酶和茉莉酸甲酯之间的相互作用。经过荧光光谱法的研究表明,超氧化物歧化酶和茉莉酸甲酯的相互作用是一个静态猝灭的过程,它们之间是以氢键和范德华力结合的,同时得到超氧化物歧化酶和茉莉酸甲酯的结合位点数n,表观结合常数K和热力学参数ΔH,ΔG,ΔS。而根据非放射性能量转移的F rster理论和同步荧光光谱证明茉莉酸甲酯和超氧化物歧化酶的相互作用位点同时接近超氧化物歧化酶色氨酸(Trp)和酪氨酸(Tyr)残基。
The interaction between CuZn-superoxide dismutase（SOD） and methyljasmonate（MeJA） was studied by fluorescence and UV-Vis spectroscopic techniques.The quenching mechanism of fluorescence of MeJA by SOD was discussed to be a static quenching procedure.The number of binding sites n and apparent binding constant K was measured by fluorescence quenching method.The thermodynamics parameter ΔH,ΔG,ΔS were also calculated.The results indicate the binding reaction was both entropy-driven and the enthalpy-driven,and the electrostatic and hydrophobic binding played major role in the interaction. The binding sites of MeJA with SOD were found to approach the microenvimnment of both Tip and Tyr by the synchronous fluorescence spectrometry. The distance r between donor （SOD） and aeeeptor （MeJA） was obtained according to FSrster theory of non-radioactive energy transfer.
出处
《分析试验室》
CAS
CSCD
北大核心
2010年第1期5-9,共5页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(20777094
30600400)项目资助
