摘要
【目的】探讨茉莉酸甲酯(MeJA)对采后香蕉果实耐冷诱导的效果及其对活性氧代谢、Ca2+-ATPase活性的影响。【方法】香蕉果皮用10μmol·L-1MeJA处理1min,在20℃恒温培养1d(取样作第0天),然后置于7℃下离体培养10d,测定果皮冷害指数、细胞膜透性、过氧化氢和超氧阴离子含量、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、NADPH氧化酶以及Ca2+-ATPase活性的变化。【结果】与对照香蕉果皮相比,10μmol·L-1MeJA处理的冷害指数较低、细胞膜透性增加缓慢;MeJA处理诱导了果皮H2O2含量和产生速率的提高,并维持了前4d的高水平,而MeJA处理降低了CAT和APX的活性,并在前4d维持了较低的水平;研究还发现,MeJA处理还诱导了NADPH氧化酶和Ca2+-ATPase活性的升高。【结论】MeJA诱导的活性氧猝发可能作为信号分子参与诱导了香蕉果皮冷害和耐冷诱导,并可能与钙信号相关。
[Objective] The effects of methyl jasmonate （MeJA） on cold resistance, reactive oxygen species （ROS） metabolism and Ca^2＋-ATPase activity of postharvest banana fruit were investigated in this paper. [Method] Banana peels were treated with 10 μmol·L^-1 MeJA for 1 min and incubated at 20℃ for 1 day （sampled as Day 0）, and then incubated in vitro at 7℃ for 10 days. The chilling injury index, cell membrane permeability, contents of H2O2 and O2, changes in activities of CAT, APX, NADPH oxidase and Ca^2＋-ATPase were determined. [Result] Compared with control peels, the chilling injury index of MeJA treated peels was less and the increase of cell membrane permeability was slower. Moreover, MeJA treatment induced the increase of H2O2 content and O2 production and maintained their high levels in early 4 days. However, MeJA treatment reduced the activities of CAT and APX and maintained low activity in early 4 days. MeJA treatment was also found to induce the activities of NADPH oxidase and Ca2^＋-ATPase of banana peels. [Conclusion] The reactive oxygen species induced by MeJA might serve as signal moleculars and be involved in chilling injury and chilling resistance of banana peels, and they might also induce calcium signaling.
出处
《中国农业科学》
CAS
CSCD
北大核心
2008年第4期1165-1171,共7页
Scientia Agricultura Sinica
基金
国家自然科学基金项目(30471219)
广东省联合基金重点项目(U0631004)
广东省自然科学基金团队项目(06200670)
关键词
茉莉酸甲酯
冷害
活性氧
香蕉果皮
Methyl jasmonate
Chilling iniury
Reactive oxygen species
Banana peel
