摘要
目的：研究缺锌对胸腺发育及Ｔ淋巴细胞活化与增殖功能的影响。方法：建立大鼠缺锌（ＺＤ）模型，测定胸腺肽、胸腺激素活性、胸腺Ｔ淋巴细胞转化、活化Ｔ淋巴细胞钙离子和活性钙调蛋白等。结果：１．ＺＤ组的血清、毛、胸腺细胞锌含量低于对照（ＡＬ）组（Ｐ＜０．０１）。２．ＺＤ组的胸腺重量、指数和细胞大小低于ＡＬ组（Ｐ＜０．０１或Ｐ＜０．０５）。３．ＺＤ组胸腺的胸腺肽含量、胸腺素活性低于ＡＬ组（Ｐ＜０．０１）。４．ＺＤ组胸腺细胞的ＤＮＡ、ＲＮＡ和蛋白质含量低于ＡＬ组（Ｐ＜０．０１）。５．ＺＤ组胸腺细胞中增殖期细胞（Ｓ＋Ｇ２／Ｍ）、增殖指数（ＰＩ）低于ＡＬ组（Ｐ＜０．０１）。６．ＺＤ组胸腺细胞内ｃＡＭＰ含量与ｃＡＭＰ／ｃＧＭＰ比值高于ＡＬ组（Ｐ＜０．０１）。７．ＺＤ组胸腺细胞内的Ｃａ２＋、ＣａＭ、ＩＬ－２、ＩＬ－２Ｒα及Ｔ细胞增殖率低于ＡＬ组（Ｐ＜０．０１）。８．ＺＤ组胸腺Ｔ淋巴细胞内的锌离子与Ｃａ２＋、ＣａＭ、ＩＬ－２、ＩＬ－２Ｒα、Ｔ淋巴细胞增殖率呈正相关（Ｐ＜０．０１）；Ｃａ２＋与ＩＬ－２、ＩＬ－２Ｒα呈正相关（Ｐ＜０．０１）；ＣａＭ与ＩＬ－２、ＩＬ－２Ｒα呈正相关（Ｐ＜０．０１）。结论：适量锌有促进胸腺发育、胸腺激素活?
The model of zinc deficiency (ZD) was estabilished in rats to investigate the effects of ZD on thymus development and the activating and proliferative functions of T lymphocytes. The findings were as follows: 1 The contents of zinc in serum, hair and thymocytes in ZD group were significantly lower than those in AL group (P<0.01,P<0.05); 2 The weight, indices and cell size of thymus in ZD group were lower than those in AL group (P<0.01,P<0.05); 3 The production of thymic peptides and thymin activity in ZD group were lower than those in AL group (P<0.01); 4 The cellular contents of DNA, RNA and protein in thymocytes in ZD group were lower than those in AL group (P<0.01); 5 The total number in S+G 2/M phase and proliferative index of thymocytes in ZD group were lower than those in AL group (P<0.01); 6 The levels of cAMP and cAMP/cGMP ratio in thymocytes in ZD group were higher than those in AL group; 7 The levels of Ca 2+ ,CaM,IL 2 and IL 2Rα in thymocytes and proliferative index of T lymphocytes in ZD group were lower than those in AL group (P<0.01); 8 In ZD group, the intracellular concentration of zinc in T lymphocytes of the thymus was positively correlated to the levels of Ca 2+ , CaM, IL 2 and IL 2Rα and the proliferative index of T cells (P<0.01). Among those indices in thymocytes, both Ca 2+ and CaM were positively correlated with IL 2 and IL 2Rα (P<0.01); These findings suggest that adequate amount of zinc can promote the development of thymus, production of thymic peptide and thymin activity, and modulate the function of T lymphocytes through affecting the concentration of Ca 2+ , level of activated CaM, contents of cAMP and cGMP and production of IL 2 and IL 2Rα in activated T lymphocytes.
出处
《营养学报》
CAS
CSCD
北大核心
1998年第3期303-307,共5页
Acta Nutrimenta Sinica
关键词
缺锌
胸腺发育
T淋巴细胞
活性
儿童
zinc deficiency Thymus development activated T cell
