摘要
目的:采用HPLC法测定紫地宁血散中没食子酸的含量。方法:色谱柱为Diamonsil C18(4.6 mm×200 mm,5μm),流动相为乙腈-含0.1%三乙胺的0.1%磷酸溶液(1∶99),流速:1.0 mL.min-1,检测波长为273 nm。结果:线性范围为0.051～0.612μg,r=1.000 0。平均回收率为99.3%,RSD为1.2%。结论:本法简便、快速、准确,可用于产品的质量控制。
Objective： An HPLC method was established for the determination of Gallic Acid in Zidiningxue San. Methods： A Diamonsil C18 （4. 6 mm ×200mm,5μm） column was used with Acetonitrile and 0. 1% phosphoric acid containing 0. 1% triethylamine （ 1 ： 99 ） as the mobile phase, with flow rate 1.0 mL · min^ -1 and the detection wavelength 273 nm. Results：The linear range was 0. 05μg - 0. 6121μg, r = 1. 000 0. The average recovery was 99.3% and the RSD was 1.2%. Conclusion：The method was convenient, sensitive and accurate. So it could be used for quality control.
出处
《中国药品标准》
CAS
2009年第6期446-448,共3页
Drug Standards of China
