摘要
对我国东南沿海日本囊对虾的4个养殖群体广东群体(GD)、台湾群体(TW)、福建群体(FJ)和浙江群体(ZJ)的线粒体细胞色素b基因片段进行PCR扩增,对产物进行测序后分析。经比对获得552bp的核苷酸序列,发现了52个变异位点,得到了50种单倍型。广东、台湾、福建和浙江群体的核苷酸多样性依次分别为0.0082、0.0044、0.0137、0.0045,各群体均存在较好的单倍型多态性和核苷酸多态性。群体遗传分化分析表明各群体间保持着一定的遗传差异,其中福建群体与其它群体之间存在明显的遗传分化。采用MEGA软件计算了群体间的遗传距离,福建群体与广东群体间的遗传距离最远0.012,台湾群体与浙江群体间的遗传距离最小为0.005。以其它4种对虾为外群构建了4个群体的NJ系统进化树,结果显示台湾群体与浙江群体最先聚为一组群。
To investigate the variation of mitochondrial cytb sequence and assess the genetic structure and polymorphism of four cultured stocks of Marsupenaeus japonicus, M. japonicus were sampled in South China Guangdong （GD）, Taiwan （TW）, Fujian （FJ）, and Zhejiang （ZJ） provinces. With PCR technique, we amplified the mt-DNA cytb gene fragment among the four stocks. 552bp nucleotide sequences of partial cytb were obtained, of which 52 variable sites and 50 haplotypes were detected. Nucleotide diversities in GD, TW, FJ, and ZJ stocks were 0.0082, 0.0044, 0.0137, and 0.0045 respectively. All the stocks showed high diversity in nucleotide and haplotype. Genetic difference in all the four stocks was found, of which the one between FJ stock and other three stocks was significant, shown by Fst analysis. The Kimura-2-paramter genetic distance calculated by MEGA software between FJ and GD stocks is the greatest at 0.012, while that between ZJ and TW ones is the smallest at 0.005. A NJ phylogenetic tree was constructed using four penaeidae shrimp as out group. These results shall be very important and useful for making scientific strategy for the family selective breeding with molecular marker assistance.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2009年第6期786-792,共7页
Oceanologia Et Limnologia Sinica
基金
浙江省重大科技专项重点项目
2006C12010号
国家科技支撑计划项目
2007BAD43B08
浙江省重大科技专项重大项目
2007C02001号
