摘要
目的：应用高效液相色谱法（high performance liquid chromatography,HPLC）测量儿童全唾液富酪蛋白浓度,分析全唾液富酪蛋白浓度与患龋状况之间的关系。方法：采集20名4～6岁龋病高危组（Caries-Susceptible,CS组,dft≥5且CSI≥10）及23名无龋组（Caries-Free,CF组,dft=0,CSI=0）儿童的非刺激性及刺激性全唾液,应用高效液相色谱法及氨基酸组成分析法对全唾液中的富酪蛋白进行测定,分析富酪蛋白浓度与患龋状况的关系。结果：CS组的非刺激性全唾液浓度低于CF组（P〈0.05）,而两组的刺激性全唾液富酪蛋白浓度无显著性差异。唾液富酪蛋白浓度与dft、dfs及CSI值无相关关系。结论：龋病高危组的非刺激性全唾液富酪蛋白浓度低于无龋组,提示富酪蛋白具有一定的抗龋作用;全唾液中的富酪蛋白浓度与dft、dfs、CSI之间无相关关系。
Objective： To study the correlation between the level of statherin in whole saliva of children and caries. Methods： 43 healthy children aged 4-6 years old were selected and divided into caries-susceptible group （CS group, dft≥ 5 and CSI≥10, n= 20） and caries- free group （CF group, dft = 0, CSI = 0, n = 23 ）. Both unstimulated and stimulated whole saliva were collected. Statherin in the saliva was identified and quantified by high performance liquid chromatography （HPLC） method with photo-diode-array detector （DAD） and amino-acid analysis method. Results： The CF group had a significantly higher concentration of statherin in unstimulated whole saliva（USWS） than that of CS group,but there were no difference of stimulated whole saliva（SWS） between CS and CF groups. There were no correlation between the concentration of statherin and the dft, dfs or CSI scores. Conclusion： The concentraion of statherin in unstimulated whole saliva of CS group was lower than that of CF group. There was no correlation between the statherin concentration in whole saliva and dft,dfs index or CSI scores.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2009年第6期778-780,共3页
Journal of Oral Science Research
关键词
儿童
全唾液
富酪蛋白
龋病
高效液相色谱法
Children Whole Saliva Statherin Dental caries High performance liquid chromatography
