摘要
目的光动力治疗是一种有效的局部肿瘤治疗手段，包括光敏剂的注射及激光照射两部分；观察以HMME作为光敏剂，再辅以630nm二极管激光照射对人肝细胞癌细胞HepG2的光动力杀伤效应。方法应用MTT技术来检测HepG2细胞的抑制率；应用AnnexinV／PI双染色流式细胞仪来检测凋亡细胞所占的百分比；两种方法被用来检测细胞凋亡，TUNEL及激光共聚焦显微镜摄片。结果在体外PDT实验中，不同的激光能量及药物浓度对HepG2细胞显示出了很强的细胞毒性（P〈0．05），并且HMME主要以凋亡的形式介导HepG2细胞死亡。结论笔者的研究证明，应用HMME及二极管激光器可以诱导人肝细胞癌细胞系HepG2发生凋亡。这种治疗手段对临床上的HCC病人有较好的临床前景。
Objective Photodynamic therapy （PDT） is an effective local cancer treatment, in which a photosensitizer is administered and the tumor is irradiated with light. We examined the effect of PDT using HMME as the photosensitizer, and the 630nm diode laser on human hepatocellular carcinoma cell line HepGz. Methods HepG2 cell line was used in all experiments. Cell viability was determined by MTT assay. The percentage of apoptotic cell was determined by flow cytometry following annexin V/PI staining. Two methods were used for the determination of apoptosis： terminal deoxynucleotidyl transferase （TdT） mediated dUTP nick end labeling assay and laser scanning confocal micro scope detection. Results In vitro PDT showed excellent cytotoxicity that was a function of laser energy and drug concentration to the HepG2 cell lines （P〈0.05）. PDT-mediated cell death occurred predominantly by apoptosis in vitro. Conclusion PDT using HMME and the diode laser induces apoptosis in human hepatocellular carcinoma cell lines. It is expected that this therapy will be clinically useful for the treatment of patients with hepatocellular carcinoma.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2009年第12期924-927,共4页
Chinese Journal of Hepatobiliary Surgery
关键词
癌
肝细胞
血卟啉单甲醚
光动力治疗
凋亡
Carcinoma hepatocellular
Hematoporphyrin monomethyl ether
Photodynamic therapy
Apoptosis
