摘要
为了研究二氧化钛在免疫隔离技术中的应用,采用溶胶-凝胶法在不同烧结温度下,以多孔钛为基底制备出金红石和锐钛矿两种不同晶型的二氧化钛（TiO2）膜,并将大鼠下丘脑神经细胞接种到TiO2膜上,并通过体外培养观察这两种晶型的TiO2对神经细胞生长的影响.实验结果显示,在金红石型TiO2膜上生长的神经细胞不仅具有典型的神经细胞结构,而且能正常分泌β-内啡肽,使细胞外液中的β-内啡肽含量始终维持在42.5-45.0 pmol/L;体外培养的第7天,神经细胞经台盼蓝染色检测其存活率为86.7%,并且细胞突起交错成网;但是在锐钛矿型TiO2膜上生长的下丘脑神经细胞则多数死亡,其存活率仅为5.3%,而少量存活的细胞不仅没有典型的神经细胞结构,而且逐渐丧失了分泌内啡肽的功能.对比结果显示金红石型TiO2比锐钛矿型TiO2更有利于神经细胞的生长.
The Effect of TiO2 membranes with different phase composition on the adherent cells growth were observed by hypothalamie neurons culture in vitro for the immunoisolation application of TiO2 membranes. The TiO2 membranes with different phase composition supported on porous Ti planar were prepared under different sintering temperature by sol - gel technique. The results showed that the hypothalamic neurons could grow well on the TiO2 membranes with rutile phase. These neurons not only had typical nerval morphological characters, but also kept its secretory function of β - endorphin. The contents of β - endorphin remained between 42.5 pmol/L and 45.0 pmol/L. The livability of neurons, which were cultured on the TiO2 with rutile phase, was 86.7%. At the same time, the hypothalamic neurons, which were cultured on the TiO2 membranes with anatase phase, mostly died and the surviving neurons （5.3 % ） had neither normal nerval morphological characters nor secretory function. These results indicated that the TiO2 membranes with rutile phase were more propitious to adherent cells growth than that with ruffle phase.
出处
《膜科学与技术》
CAS
CSCD
北大核心
2009年第6期81-84,共4页
Membrane Science and Technology
