摘要
目的探讨过氧化物酶体增殖激活物受体γ(PPARγ)配体对肾癌细胞生成血管内皮生长因子(VEGF)的影响。方法通过RT-PCR及Western blot观察TZDs处理后786-O和A498肾癌细胞表达VEGF的能力。结果50μmol/L的TZDs抑制肾癌细胞VEGF基因和蛋白的表达。结论激活PPARγ可以抑制肾癌细胞的血管生长,PPARγ有可能成为肾细胞癌新的治疗靶点。
Objective To investigate the expression of vascular endothelial growth factor （VEGF） induced by peroxisome proliferator-activated receptor y （PPART） in renal cell carcinoma （RCC） derived cell lines. Methods RT-PCR was performed to determine the VEGF mRNA before and after treatment with 0 and 50μmol/L TZDs （pioglitazone or troglitazone） for 48 h in two RCC-derived cell iines （786-O and A498）. The mutative expressions of VEGF protein were detected by western blot analysis. Results Treatment with 50μmol/L TZDs （for 48 h） decreased both the VEGF mRNA and protein expression in RCC-derived cells. Conclusion The results reveal that PPARy ligands involve angiogenesis inhibition on RCC ceils. PPARγ may be the candidates target for a novel approach to the treatment of RCC.
出处
《福建医药杂志》
CAS
2009年第6期6-8,共3页
Fujian Medical Journal
基金
福建省科技人才创新项目(2005J072)
