摘要
目的：为了探索大面积Ⅲ度烧伤混合植皮创面愈合过程中生长因子的作用。方法：本实验以大鼠为模型，在异体皮移植后３、５、７、１４、２１和２８天以及自体皮移植后２、４、１１、１８和２５天分别取自体皮区、异体皮区皮肤，用酸性硫氰酸胍苯酚氯仿（ＡＧＰＣ）法提取总ＲＮＡ，经逆转录聚合酶链反应（ＰＴＰＣＲ），测定ＴＧＦα、ＴＧＦβ１、ＰＤＧＦ和ｂＦＧＦ的ｍＲＮＡ表达量。结果：１）这四种生长因子ｍＲＮＡ表达在自体皮区及异体皮区各时相都能被检测到，并且自体皮区表达均高于异体皮区。２）在自体皮区，ＴＧＦα的表达在２大以后持续升高，表达峰值在１８天；ＴＧＦβ１在２天就有高表达，第二峰值出现在１８天；ＰＤＧＦ在２天有较高表达，表达峰值在４天，随后逐渐下降，２５天回升：ｂＦＧＦ也在２天有很高表达，以后下降，象ＰＤＧＦ一样，２５天回升。３）在异体皮区，创面愈合早期这四种生长因子表达都较低，以后各有不同程度升高。结论：１）移植的自体皮与异体皮都存活，并反映了自体皮区的愈合再生较异体皮区旺盛。２）在自体皮区这四种生长因子通过自分泌作用，旁分泌作用，到达创伤区，沿着信号转导通路，相互协同发挥效应，促进创面愈合。３）在异体皮区存在排异反应，?
Objective: To scrutinize the effect of growth factors resulting from the intermingled skin graft on wound healing. Methods:By means of RT-PCR technique, mRNA expressions of TGFα, TGFβ1, PDGF and bFGF of autografts and allografts on burnt rats were quantitatively analysed. Results:(1) The mRNA expressions of four growth factors mentioned above were found both in allografts and autografts, the expressions being higher in autografts than in allografts; (2) In autografts, TGFα expression began to rise from second post-transplant day(D2), reaching a peak on D18; TGFβ1 expression reached a high level on D2 and had its peak on D18; PDGF expression had its peak on D4, rebounded on D25; bFGF expresion, exceedingly high on D2, dropped and rebounded in the same manner as in PDGF; (3) In the early stage of wound healing, The mRNA expression of all four growth factors were lower in allografrs, roes up in varying degrees thereafter. Conclusions: (1) Graft survival took place both in autografts and allografts with the former performing better than the latter; (2) The combinaction of the growth factors that reached the affected area through autocrine and paracrine secretion probably promoted the healing of the burnt wound following signal transduction channels; (3) Rejection, which occurred in allografts was responsible for the decreaed viability of allografts and their replacement by the host skin.
出处
《上海医学》
CAS
CSCD
北大核心
1998年第9期516-518,共3页
Shanghai Medical Journal
基金
国家自然科学基金
关键词
烧伤
混合移植
生长因子
皮肤移植
MRNA
Wound healing Intermingled skin transplant Growth factor PT-PCR mRNA
