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番茄中性/碱性蔗糖转化酶基因的电子克隆、分析及表达载体的构建 被引量：7

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摘要 利用电子克隆方法从番茄中分离出番茄中性/碱性蔗糖转化酶基因部分cDNA序列。测序结果表明该序列长度为1718bp,包含完整的开放阅读框,编码571个氨基酸。Alignment分析显示该蛋白与其他植物已知的中性/碱性蔗糖转化酶具有很高的同源性,推测该蛋白为番茄中性/碱性蔗糖转化酶。RT-PCR检测显示它在蕾、果、叶中的表达强于在花、茎中的表达,胁迫处理下表达基本一致。
作者 高媛媛 杨郁文 张保龙 倪万潮
机构地区 江苏省农业科学院农业生物技术研究所
出处 《江苏农业科学》 CSCD 北大核心 2009年第6期36-38,共3页 Jiangsu Agricultural Sciences
基金 江苏省自然科学基金(编号:2007125)
关键词 蔗糖转化酶 番茄 电子克隆
分类号 Q785 [生物学—分子生物学]
  • 相关文献

参考文献15

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二级参考文献37

共引文献100

同被引文献80

  • 1潘秋红,张大鹏.植物转化酶的种类﹑特性与功能[J].植物生理学通讯,2004,40(3):275-280. 被引量：25
  • 2乔永旭,刘栓桃,赵智中,邢国明,何启伟.甜瓜果实发育过程中糖积累与蔗糖代谢相关酶的关系[J].果树学报,2004,21(5):447-450. 被引量：54
  • 3沈火林,朱鑫,冯锡刚,刘燕燕.芹菜耐寒性的初步鉴定[J].中国农学通报,2006,22(2):316-319. 被引量：9
  • 4陈俊伟,秦巧平,谢鸣,蒋桂华,徐红霞,程建徽,吴江,孙崇波.草莓果实蔗糖和己糖的代谢特性及其与糖积累的关系(英文)[J].果树学报,2007,24(1):49-54. 被引量：11
  • 5Sturm A, Chrispeels M J. eDNA cloning of carrot extracelhdar beta- fructosidase and its expression in response to wounding and bacte- rial infection [ J ]. Plant Cell, 1990,2 : 1107-1119.
  • 6Murayama S, Handa H. Genes for alkaline/neutral invertase in rice:alkaline/neutral invertases are located in plant mitochondria and also in plastids [ J ]. Planta,2007,225 : 1193-1203.
  • 7Gallagher J A, Pollock C J. Isolation and characterization of a cD- NA clone from Lolium temulentum L. encoding for a sucrose hydro- lytic enzyme which shows alkaline/neutral invertase activity [ J ]. Journal of Experimental Botany, 1998,49:789-795.
  • 8Lammens W, Le R K,van L A, et al. Crystal structures of Arabidop- sis thaliana cell-wall invertase mutants in complex with sucrose [ J ]. Journal of Molecular Biology,2008,377 : 378-385.
  • 9Vargas W A, Pontis tt G, Salerno G L. Diftrential expression of al- kaline and neutral in,Jerlases in response to environmental stresses : characterization of an alkaline isoform as a stress-response enzyme in wheat leaves [J]. Planta ,2007,226 : 1535-1545.
  • 10Vorster D J, Botha F C. Partial purification and characterisation of sugarcane neutral invertase [ J ]. Phytochemistry, 1998, 49: 651-655.

引证文献7

二级引证文献31

江苏农业科学
2009年 第6期

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