摘要
本文报道了球形芽孢杆菌、苏云金芽孢杆菌和森田芽孢杆菌三种昆虫病原菌的超氧化物歧化酶（SOD）的电泳图型及其同源性。实验证明B．S和B．tSOD分别具有Ef25～30、56．8和52．9特征酶带，B．mSOD也具有与B．t相同的特征酶带；免疫琼脂双扩散实验证明B．sSOD与B．t和B．mSOD无同源性，B．tSOD抗血清与B.sSOD抗原无沉淀反应，但与B．mSOD抗原产生沉淀反应，证明B．s与B.t和B．m的亲缘关系远，B．t和B．m的亲缘关系近。
The PAGE patterns and homogeny of Superoxide dismutase (SOD) of Bacillus sphaericus (B.s), Bacillus thuringiensis (B.t) and Bacillus moritai (B.m) were reported in this paper. It was found out that SOD of B.s and B.t were possessed of special PAGE bands of Ef 25～30, Ef 56.8 and Ef 52.9 respectively. The special bands of SOD of B.m were the same as that of B.t. The results of immunodiffusion showed that SOD betWeen the toxic strains and non-toxic strains of B. s were homogenous, and however those between B.s and B.t were not homogenous. The experiments also showed all SOD of tested 18 B.t strains and B.m strain were possessed of homogenies.
出处
《微生物学通报》
CAS
CSCD
北大核心
1998年第5期249-252,共4页
Microbiology China
基金
中国科学院区系分类特别支持项目
关键词
超氧化物歧化酶
同源性
电泳图型
芽胞杆菌
Bacillus sphaericus, Bacillus thuringiensis,Bacillus moritai, Superoxide dismutase,Homogeny
