摘要
本文介绍非培养细胞贴壁的简易方法和细胞内Ｃａ２＋观察。以ｆｌｕｏ３／ＡＭ进行染色，在３７℃孵育箱内孵育６０分钟，用激光共聚焦显微镜监测细胞内Ｃａ２＋的荧光信号。结果表明本方法可成功地应用于共聚焦显微镜测定细胞的研究。
A simple method for fixing noncultured cells on glass was developed.The intracellular free calcium was measured with the confocal microscopy.The cells were incubated with fluo3/AM at 37℃ for 60 minutes.Fluoresence singal of free calcium was measured under the laser scanning confocal microscopy.The results showed that it was able to successfully measure cells with the confocal microscopy.
出处
《激光生物学报》
CAS
CSCD
1998年第3期220-222,共3页
Acta Laser Biology Sinica
