摘要
用细胞培养及trkA免疫组化染色方法，观察了坐骨神经外植块及巨噬细胞和雪旺氏细胞条件培养基对胚鼠脊神经节感觉神经元高亲和力神经生长因子受体表达的影响。结果显示：各组感觉神经元均产生免疫阳性反应，雪旺氏细胞条件培养基和巨噬细胞条件培养组免疫反应强度与对照组相同，联合培养组免疫反应为强阳性，与巨噬细胞条件培养组相比差别高度显著（P＜0.005）。免疫反应阳性神经元可分为2类：一类形状为圆形或椭圆形，另一类为多角形。实验组神经元和非神经元生长状况明显优于对照组。本研究结果提示：坐骨神经的溃变轴突有促进脊神经节内感觉神经元trkA表达的作用;雪旺细胞条件培养和巨噬细胞条件培养组的某些因子可直接或间接地维持感觉神经元的生存，但不促进其trkA的表达。
The effects of the explants of sciatic nerve, MΦCM and ScCM on the HNGFR expression in sensory neuron of spinal ganglion were observed by cell culture and trkA immunostaining. The results showed that trkA positive immunoreactivity appeared in sensory neurons of each group. The MΦ CM and ScCM groups were similar to the control group in positive immunoreactivity. In coculture group this immunoreactivity became stronger. There was significant difference as compared with MΦ CM group (P<0. 005). Immunoreactive neurons were divided into two types. One was round or ellipse, another was multiangular.The growth of neuron and nonneuron in coculture MΦ CM and ScCM group was better than that in control group. The present results indicate that degenerative axons in sciatic nerve promoted expression of trkA in sensory neuron of spinal ganglion. Certain factors in ScCM and MΦ CM maintained direct or indirect sensory neuronal survival, but did not promote trkA expression.
出处
《神经解剖学杂志》
CSCD
北大核心
1998年第3期247-250,共4页
Chinese Journal of Neuroanatomy
