摘要
真核细胞基因组ＤＮＡ的甲基化主要发生在ＣｐＧ二核苷酸对的胞嘧啶环上（ｍ５Ｃ）［１，２］．在真核细胞基因组ＤＮＡ内，约７０％的ＣｐＧ位点发生了甲基化．ＣｐＧ位点在基因组ＤＮＡ内并不是均匀分布的，多数聚集在一些基因的５’端．大量的实验证据表明，真核基因５...
As one importent member of human heat shock proteins,human hsp 90β gene is constitutively experssed in various tissues under physiological condition and it is highly inducible at elevated temperature.A study was carried out to test the methylation status of some CpGs upstream of this gene in Jurkat cells under physiological and heat shock conditions.Extracted genomic DNA from Jurkat cells under both conditions were cut by methylation sensitive restricted endonuclease (Msp Ⅰ/Hpa Ⅱand Hha Ⅰ)and hybridized with human hsp90β cDNA probe.Several CpG dinucleosides sites between -1 102 bp and -173 bp were found non Methylated before and after heat shock treatment.The study indicates that keeping CpG sites upsteam of human hsp90β gene at non methylated status may be needed for the constitutive expression of this gene.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1998年第5期632-635,共4页
Chinese Journal of Biochemistry and Molecular Biology
