摘要
目的:探讨补体在大鼠大脑缺血/再灌注(ischemia-reperfusion,I/R)损伤中的作用及重组人可溶性补体受体Ⅰ型SCR15-18蛋白(sCR1-SCR15-18)的保护作用。方法:75只雄性SD大鼠,随机分为假手术组、I/R组和sCR1-SCR15-18保护组。采用线栓法建立大鼠大脑中动脉闭塞模型(middle cerebral artery occlusion MCAO),缺血2h,再灌注24h后,进行神经功能学评分,测定脑梗死体积、大脑皮质髓过氧化物酶(myeloperoxi-dase,MPO)活性,观察大脑皮质区补体C3b沉积和病理改变。结果:缺血/再灌注24h后,sCR1-SCR15-18保护组神经功能学评分,脑梗死体积及脑皮质MPO活性明显低于I/R组(P<0.05);sCR1-SCR15-18保护组缺血脑组织补体C3b沉积明显减少,病理损伤减轻。结论:补体在脑I/R损伤中起一定作用,sCR1-SCR15-18蛋白对大鼠I/R损伤脑具有保护作用。
AIM： To explore the effect of complement on the cerebral ischemia/reperfusion injury in rat and the protection by sCRI -SCR15 -18. METHODS： 75 male SD rats were randomly divided into three groups： sham operation group （SO, n = 15）, middle cerebral artery occlusion and reperfusion （MCAO） without treatment group （I/R, n = 30） ; MCAO treated with sCR1 - SCR15 - 18 group （sCR1 - SCR15 - 18, n =30）. After the MCAO for 2h, then reperfusion for 24 h, the scores of neural behavioral functional deficits were determined. Infarction area was measured by TTC staining. Activity of MPO in cerebral cortex was detected. C3b deposition and pathological change were observed by immunohistochemial staining and HE staining, respectively. RESULTS： After reperfusion for 24 h, the neurological deficits score, infarction area and activity of MPO in sCR1 - SCR15 - 18 group were decreased compared to I/R group. In sCR1 - SCR15 - 18 group, C3b deposition in ischemic area was decreased and pathological injury was improved compared to I/R group. CONCLUSION： Complement plays a role in cerebral ischemiareperfusion injury and sCR1 -SCR15 -18 exerts a protective effect by inhibiting the excessive activation of complement.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2009年第12期2436-2440,共5页
Chinese Journal of Pathophysiology
基金
重庆市自然科学基金资助项目(No.2007BB5011)
国家自然科学基金资助项目(No.30471723)
