摘要
目的：建立以表面活性剂为抑制剂的匀相法测定血清低密度脂蛋白胆固醇。方法：用HLB值为13.2的混合表面活性剂将血清标本中的HDL-C、VLDL-C、CM等脂蛋白组分特异性清除后,仅使LDL-C裂解并参加胆固醇反应。结果：该法的线性范围达12.00 mmol/L;回收率为96.2%～103.7%;批内变异系数（CV）为0.65%～2.88%,批间变异系数（CV）为1.25%～3.56%;与参考方法相关良好,回归方程及相关系数分别为Y=0.9944X＋0.0510;HDL-C〈2.8 mmol/L、TG〈12 mmol/L、胆红素〈320 mg/L、血红蛋白〈14 g/L不干扰本法测定。结论：本法实现了以表面活性剂为清除剂的LDL-C匀相法直接测定,在测定过程中无浊度产生,也不堵塞自动分析仪的管道,具有良好的精密度和准确性,适合临床推广应用。
Objective：To establish a new method of homogenous assay for LDL-C using mixed surfactants as selective reagent.Methods：After the LDL-C in serum was inhibited by the mixed surfactants,the serum CM,VLDL-C,HDL-C were hydrolyzed in the first step,the second step LDL-C was hydrolyzed and reacted to the cholesterol reagent.Results：The linearity of this method was up to 12.00 mmol/L;The recoveries ranged from 96.2%~103.7%;The within-run CV ranged from 0.65%~2.88%,The between-run CV ranged from 1.25%~3.56%;Comparing this method（Y） with reference method,we obtained the regression equation： Y=0.9944X＋0.0510;No interference were showed when addition of isolated HDL ftaction（HDL-C up to 2.8 mmol/L） and VLDL fraction（VLDL-TG up to 12mmol/L）,bilirubin（320 mg/L） and hemoglobin（14 g/L） to pooled serum,represently.Conclusion：During the homogenous process of LDL-C determination using mixed surfactants as selective reagent,there was no turbidity production in the reaction system,and did not stop up the pipeline of the autoanalyzer.The method had good precision and accuracy,and suitable for clinical application.
出处
《中国卫生检验杂志》
CAS
2009年第12期2891-2893,共3页
Chinese Journal of Health Laboratory Technology
基金
浙江省新苗人才计划项目(2007G60G2090028)
浙江省大学生科技创新推广项目(2008年资助)
