摘要
目的建立一种新的预期用作参考方法的高密度脂蛋白胆固醇(HDLC)和低密度脂蛋白胆固醇(LDLC)准确测定方法。方法血清与含2-巯基乙醇(ME)和脯氨酸的溴化钠溶液混合,使背景密度为1．063,超速离心分离高密度脂蛋白(HDL);血清与含脯氨酸的溴化钠溶液混合,使背景密度为1．006,超速离心分离HDL和低密度脂蛋白(LDL);用高效液相色谱测定两种脂蛋白组分胆固醇。结果ME和脯氨酸可有效消除脂蛋白(a)对超速离心法分离HDL的影响;新方法测定HDLC和LDLC的总变异系数分别为0．96％～2．07％和0．65％～1．12％。结论建立血清HDLC和LDLC准确测定方法,方法可靠、精密、简便,可望用作HDLC和LDLC测定参考方法。
Objective To investigate the measurement of serum high-density lipoprotein （HDL） cholesterol （HDLC） and low-density lipoprotein （LDL） cholesterol （LDLC） by uhracentrifugation and high- performance liquid chromatography （HPLC） and to develop a reliable method for HDLC and LDLC measurements for use as a reference method. Methods Serum aliquots were centrifuged in the presence of 2-mercaptoethanol and proline at a density of 1. 063 g/ml for the separation of HDL and in the presence of proline at a density of 1. 006 g/ml for the separation of HDL and LDL. Cholesterol concentrations of the lipoprotein fractions were analyzed by HPLC. Results 2-mercaptoethanol and proline together effectively eliminated the contamination of lipoprotein （a） in HDL separated by ultracentrifugation that enabled reliable separation of lipoproteins by uhracentrifugation and precise measurement of HDLC and LDLC. The total CVs for HDLC and LDLC were 0. 96% -2. 07% and 0. 65% -1. 12% , respectively. Conclusions A new method for the measurement of HDLC and LDLC by ultracentrifugation and HPLC has been established. It is reliable, precise and simple and may be used as a candidate reference method for HDLC and LDLC measurements.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2006年第8期742-746,共5页
Chinese Journal of Laboratory Medicine
基金
部分由北京市自然科学基金项目(2042023)
国家自然科学基金项目资助(30476150)
关键词
脂蛋白类
胆固醇
超速离心法
色谱法
高压液相
Lipoproteins
Cholesterol
Uhracentrifugation
Chromatography, high pressure liquid
