摘要
采用小鼠耳廓肿胀法、大鼠足跖肿胀法和小鼠肉芽组织增生法研究治疗奶牛乳房炎的纯中药制剂"乳康散"的抗炎效果;采用改进Karber法,以半数致死量(LD50)作为评价急性毒性强度的指标,研究了"乳康散"急性毒性作用。结果表明,"乳康散"的低、中、高剂量组对小鼠耳廓的肿胀度,分别为10.53mg、8.81mg、8.33mg,与对照组(生理盐水)的肿胀度(12.42mg)相比差异显著(P<0.05);"乳康散"组的小鼠足跖肿胀抑制率分别为18.35%、20.74%、18.13%,对照组为21.67%;"乳康散"组小鼠的棉球增重分别为11.13mg、8.72mg、10.93mg,与对照组的棉球增重(12.54mg)相比差异显著(P<0.05)。在急性毒性试验中无小白鼠死亡,LD50>60000mg/kg。表明"乳康散"能有效对抗急性和慢性炎症,抗炎效果显著,且该药安全可靠,可作为临床用药。
The methods of mice ear-swelling, rat metatarsus-swelling and granulation tissue hyperplasia of mice induced by tampon were adopted to study the anti-inflammatory action of pure traditional Chinese medicine ＂Rukangsan＂ for dairy cow mastitis,and improved Karbr was adopted to study the toxic effect of ＂Rukangsan＂ with LD50 as the evaluate index of the acute toxicitystrength.The test result indicated： ＂Rukangsan＂to mice ear swell were 10.53mg, 8.8ling, 8.33mg, respectively in the three test groups（high dosage, middle dosage and low dosage of＂Rukangsan＂）. There have significant difference between the control group（l12.42mg） and test groups（P〈0.05）. Inhibition rate of＂Rukangsan＂to rat metatarsus-swelling were 18.35%, 20.74%, 18.13%, respectively, while it was 21.67% in the control group. Mice granulation tissue hyperplasia in ＂Rukangsan＂ groups were 11.13mg, 8.72mg, 10.93mg, respectively. There have significant difference between the control group （12.54mg） and test groups（P〈0.05）. In the actute toxicity test, none mouse is dead, so the LD50 of ＂Rukangsan＂ injected by mice abdominal cavity were more than 60 000mg/kgBW. The results showed that the anti-inflammation action of＂Rukangsan＂was significantly for both of active, and chronic inflammation period.＂Rukangsan＂ belongs to an practical innoxious substance and can be used in the clinical research.
出处
《中国奶牛》
2009年第12期46-49,共4页
China Dairy Cattle
关键词
中药复方制剂
小鼠
抗炎试验
急性毒性试验
Cow mastitis
Chinese herhal medicine prescription
Anti-inflammatory test
Acute toxicity test
