摘要
对浙江省四个主要奶牛养殖区金华、杭州、宁波以及台州的30个奶牛场中846头泌乳牛的3178个乳区进行了奶牛隐性乳房炎病原菌调查。首先用HMT乳房炎诊断试剂检查乳区奶样,采集HMT反应为阳性(++)和强阳性(+++)的乳区奶样进行细菌分离鉴定,对分离到的金黄色葡萄球菌(Staphylococcus aureus)进行药敏试验。结果表明,浙江省奶牛隐性乳房炎的头阳性率为54.3%,乳区阳性率为28.0%。主要病原菌的检出率依次为凝固酶阴性葡萄球菌(Coagulase negative staphylococci)34.0%、无乳链球菌(Streptococcus agalactiae)27.0%、金黄色葡萄球菌(Staphylococcus aureus)12.2%、停乳链球菌(Streptococcus dysgalactiae)13.0%、乳房链球菌(Streptococcus uberis)6.2%等。药敏试验结果表明,对青霉素、氨苄青霉素敏感的金黄色葡萄球菌分离株只有20.3%,对四环素和红霉素的敏感率也较低(34.8%和47.8%)。多重耐药现象比较普遍,主要是对青霉素、氨苄青霉素、四环素、红霉素同时耐药。
This study investigated pathogen bacteria causing dairy subclinical mastitis in the main regions of dairy production in Zhejiang Province. On 30 dairy farms, 3 178 quarter milk samples were collected from 846 lactating dairy cows for subclinical mastitis test using HMT. The samples with HMT reaction ＋＋ or ＋＋＋ were bacteriologically examined. Staphylococcus aureus isolated from the samples were usett for antimicrohial drug sensitivity test. The results indicated 28.0% of 3178 quarters from 54.3% of 846 cows were detected to have subclinical mastitis. The bacteria isolated from the samples were Coagulase-negative staphylococci（34.0%）, Streptococcus aga/actiae（27.0%）, S. aureus （12.2%）, Streptococcus dysgalactiae（13.0%）and Streptococcus uberis（6.2%）. Only 20.3% of S. aureus isolates were sensitive to penicillin and ampicillin and 34.8% and 47.8% of the isolates sensitive to tetracycline and erythromycin respectively. The bacteria resistant to multiple antibiotics such as penicillin, ampicillin, tetracycline and erythromycin were commonly found.
出处
《中国奶牛》
2009年第12期39-42,共4页
China Dairy Cattle
基金
浙江省科技厅科研项目
项目编号:2005C12015
关键词
浙江省
奶牛
乳房炎
病原菌
耐药
Zhejiang Province
Dairy cows
Subclinical
Mastitis
Pathogenic bacteria
Drug resistance
