摘要
目的:探讨G0S2在调节鼠骨髓基质细胞向脂肪细胞分化过程中的作用。方法:利用原代培养小鼠骨髓基质细胞(Marrow strom cell,MSC),脂质体转染法将表达质粒pCAG-PPARγ2及含报告基因的pGL2-COL1A质粒共转染至MSC,G418筛选,RT-PCR检测PPARγ及G0S2 mRNA表达,免疫组化检测PPARγ的表达,westen blot检测G0S2的表达,油红O染色检测细胞内脂滴。结果:MSC在未分化状态下不表达G0S2,经PPARγ基因转染后,G0S2开始表达,转染细胞最终分化为脂肪细胞。结论:PPARγ通过调节G0S2启动子调节后者表达,进而调节MSC向脂肪细胞分化,G0S2基因参与调节MSC向脂肪细胞分化。
Objective：To explore the effects of G0S2 in the differentiation of mouse mallow stroma cells （MSCs） into adipocytes. Methods： The primary MSCs were cultured. The eukaryotic expression plasmid vector pCAG-PPARγ2 and pGL2-COL1A reporter vector were cotransfected into MSCs with lipotransfection method followed by G418 selection. The expression levels of PPARγ mRNA and G0S2 mRNA were detected by RT-PCR. Immunohistochemistry and Westen blot were emplored to study the protein expression of PPAR γ and G0S2. Lipid droplets in the cells were stained with Oil Red O. Results： No expression of G0S2 mRNA was found in undifferentiated MSCs. The GOS2 mRNA expressed in the MSCs after transfected with pCAG-PPAR γ 2, and the cells finaly differentiated into adipocytes. Conclusion： PPARγ stimulated G0S2 promoter activity via the PPRE in vivo.Then the expressions of G0S2 can regulate the adipocyte differentiaiton of MSCs.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第11期1495-1498,共4页
Journal of Chongqing Medical University
