摘要
目的探讨不明原因复发性流产患者外周血以CD4+CD25high-intCD127lowTreg(CD4+CD25high-intCD127lowTregularcells)为代表的调节性T细胞表达及其意义。方法采用三色荧光抗体标记及流式细胞术分析20例不明原因复发性流产患者、正常妊娠组和正常非孕对照组外周血CD4+CD25high-intCD127lowTreg占CD4+T淋巴细胞的比例。结果正常孕妇外周血CD4+CD25high-intCD127lowTreg较正常对照组差异有统计学意义(P<0.01),复发性流产患者CD4+CD25high-intCD127lowTreg调节性T细胞较正常对照组差异有统计学意义(P<0.01),正常孕妇组较复发性流产患者组差别则更显著;其中流产次数小于或等于3次的患者与流产次数大于或等于4次的患者其外周血CD4+CD25high-intCD127lowTreg占CD4+T细胞百分比差异无统计学意义(P>0.05)。结论原因不明复发性流产患者外周血中调节T细胞比例降低,这种改变导致原因不明复发性流产患者细胞免疫耐受被打破,可能是原因不明复发性流产患者发病的重要原因之一。
Objective To investigate the expression and significance of CD4＋CD25high-intCD127lowTreg（CD4＋CD25high-intCD127lowT Regular Cells） in the peripheral blood（PB） from patients with unexplained recurrent spontaneous abortion（URSA）,to explore the mechanism of autoimmune intolerance in URSA.Methods The proportion of CD4＋CD25high-intCD127lowTreg in the PB of three groups of people （URSA patients that they had been diagnosed clearly,normal non-pregnant women and the normal pregnant woman） analyzed by triad colour fluoresce-labeled monoclonal antibody and using multiparameter flow cytometry.Results The proportion of CD4＋CD25high-intCD127lowTreg/T CD4＋ cells in the normal pregnant woman was significantly higher htan those in control group（P0.01）;the proportion of CD4＋CD25high-intCD127lowTreg/T CD4＋ cells in URSA patients was significantly lower than those in control group（P0.01）.Conclusion The percentages of CD4＋CD25high-intCD127lowTreg was decreased,which break down the autoimmune tolerance in URSA,these changes of CD4＋CD25high-intCD127lowTreg in number might participate in onset of URSA,it may be correlated to pathogenesis of URSA.
出处
《安徽医学》
2009年第11期1340-1342,共3页
Anhui Medical Journal
关键词
复发性流产
调节性T细胞
流式细胞术
Recurrent spontaneous abortion
Regulatory T cell
Flow cytometry
