摘要
目的:研究人参皂苷对重型脑外伤后大鼠神经细胞凋亡的保护作用及其机制。方法:健康雄性W ist-ar大鼠60只,分为正常对照组10只,未治疗组10只,人参皂苷治疗组40只。参照Feeney法制作重型颅脑损伤模型,并于术后6h、24h、48h、72h、5天、7天共6个时相点取大鼠脑组织,用TUNEL法观察凋亡细胞,并计算凋亡指数;透射电镜下观察细胞线粒体形态的改变;应用RT-PCR法检测神经细胞COX-2 mRNA的表达。结果:未治疗组脑外伤后6h出现较多TUNEL染色阳性细胞,72h达到高峰,5天后明显减少;人参皂苷治疗组各个时间点TUNEL染色阳性细胞数明显低于未治疗组。电镜下可见细胞固缩,凋亡小体形成,线粒体肿胀变形。COX-2mRNA的表达与细胞凋亡同步升高并同步下降,治疗组各个时间点COX mRNA的表达明显低于未治疗组。结论:COX-2诱导线粒体途径细胞凋亡作用在重型脑外伤后的损伤中起重要作用。人参皂苷对大鼠重型脑外伤后神经细胞凋亡有保护作用,其作用机制可能与人参皂苷抑制COX-2的表达,从而抑制COX-2诱导的线粒体途径细胞凋亡有关。
Objectives： To study the protection effects for cells apoptosis after rat severely cerebral trauma by ginsenoside and its mechanism. Methods： 60 healthy male rats were randomly divided into normal groups, non -therapy groups, and Ginsenoside -therapy groups. According to Feeney＇ methode, a severely cerebra/trauma model were made. Make frozen sections using rat brain tissue at 6 phase as 6,12,24,72hours and 5,7days after surgery. To observe eeUs apoptosis by TUNEL technique and to calculate apoptotie index. To observe the ultrastructural organization of chondriosome by electron microscope, and to detect the expression of COX - 2 mRNA by semi - quantitation RT - PCR. Results： Some TUNEL positive cells were observed at 6 hours after surgery, and increased to the leak at 72 hours and decreased obviously at the 5th day. The TUNEL positive cells in Ginsenoside - therapy group were lower than the non - therapy group at every stage. Neuronal shrinkage and apoptotic body and swelling of chondriosome could be observed by electron microscope. The expression of COX - 2 were increased and decreased same as cells, apoptosis. The expression of COX - 2 in Ginsenoside - therapy group were lower than the non - therapy group at every stage. Conclusion： The ehondriosome pathway induced by COX -2 played an important role in the damage after severely cerebral trauma. Ginsenoside could take protection for cells apoptosis after rat severely cerebral trauma. Inhibiting the expression of COX - 2, and then inhibiting the cells apoptosis induced by COX -2 might be one of the mechanism.
出处
《中华中医药学刊》
CAS
2009年第12期2554-2557,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省医药卫生科学研究基金项目(2007A141)
