摘要
本文报告了流行性出血热病毒(Epidemic haemorrhagic fever virus,EHFV)J10、A9株分别感染实验家兔后,用反向间接ELISA(RIELISA)和间接免疫荧光(IFA)方法,动态观察了家兔特异性IgG抗体水平的变化。发现特异性IgG于感染后第1周开始出现,以后抗体水平稳步上升,于第5—7周便达到最高水平,高滴度的抗体可以维持较长时间。结果还表明;RIELISA检测家兔抗体的敏感性明显优于IFA法。用IFA进行的交叉反应还证实;EHFV-J10、A9两种毒株的抗血清能与不同来源的数株病毒发生特异性结合反应。
Rabbits were inoculated with J10 and A9 strains of epidemic haemorrhage fever virus (EHFV) in order to srudy the specific antibody response during EHFV infection. The results showed that the specific IgG of low titre was detected by the reverse indirect ELISA and the indirect immunofluorescence (RIELISA and IFA) one week post-infection. The peak of IgG titres were then detected 6-7weeks post-infection and the high level of antibody was maintained for at least 13 weeks. The antiserum of the rabbits anti-EHFV J10 and A9 strains cross react with a number of strains of EHFV. The results of the experiment also indicated that RIELISA method is more sensitive in detecting the specific anibody than IFA.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1990年第2期106-108,共3页
Immunological Journal
关键词
流行性出血热
特异性抗体
病毒感染
Epidemic haemorrhagic fever virus, Rabbits specific IgG,RIELISA, IFA
