摘要
目的：探讨维生素Ａ抑制Ｓｈｏｐｅ肿瘤细胞生长的机理。方法：电镜观察Ｓｈｏｐｅ肿瘤细胞凋亡的形态学改变；琼脂糖凝胶电泳进行ＤＮＡ片段化；流式细胞仪检测凋亡细胞的百分比。结果：电镜下可见ＶＡ诱导的Ｓｈｏｐｅ肿瘤细胞变小，细胞浆固缩，染色质聚集并靠近核膜，在１．５％琼脂糖凝胶电泳中，Ｓｈｏｐｅ肿瘤细胞ＤＮＡ呈现明显的阶梯状图谱。流式细胞仪检测经１０＾－５ＭＶＡ作用２４ｈ、４８ｈ、７２ｈ后。
Objective:To study the mechanism of VA inhibted shope carinoma cells growth.Method:The mophologic changes in shope carinoma cells apoptosis were observed by eletronmicroscope.DNA fragments were prepared by agar egl eletronphorese and the percentages of apoptosis were detected by flow cytometer.Results:Under electronmicroscope:shope carcinoma cells induced by VA became small,Cytoplasm shrinked,chromosome assembled and closed to nuclear membrane.Obvious DNA ladder appeared in 1.5% agar gel electeronphores.After 24t.48h and 72h with 10-5 MVA the percentages of apoptosis were 17.1% 22.4% 32.4% respectively by flow cytometer.Conclnsion:The results showed tat VA had obvious inhibition to shope carcinoma cells.
