摘要
目的 探讨阿司匹林对胃癌细胞株SGC 790 1的细胞毒作用。方法 采用MTT法检测阿司匹林对胃癌细胞的抑制率 ,采用流式细胞仪测定细胞周期 ,采用琼脂糖凝胶电泳法检测细胞凋亡。结果 阿司匹林对SGC 790 1细胞株的抑制作用具有量效和时效依赖关系 ;可使SGC 790 1细胞的S期细胞比例和G2 /M期细胞比例升高 ,G0 /G1期细胞比例下降 ,并呈一定的剂量效应关系 ;未见典型的阶梯状凋亡条带。结论 阿司匹林对SGC 790 1细胞株有细胞毒作用 。
Objective To investigate the cytotoxicity of acetylsalicylic acid(aspirin) on the SGC-7901.Methods Cell cycle was determined with Flow Cytometry (FCM) analysis.Inhibition rate was tested by MTT method.Apoptosis was determinded by gelose electrophoresis.Results As the time and dose increased,the cytotoxicity of aspirin on SGC-7901 was significantly increased.FCM data showed that.Among phases of cell cycle,the S and G 2/M phase ratio were increased significantly and G 0/G 1 phase ratio was inhibited in a dose-dependent manner.DNA LADDER of typical apotosis was not found by gelose electrophoresis.Conclusion Aspirin has cytotoxicity on SGC-7901.It may be associated with the phases of cell cycle.
出处
《实用癌症杂志》
2001年第4期403-405,共3页
The Practical Journal of Cancer
