摘要
目的:探讨异丙酚对大鼠肠缺血再灌注后致肾损伤的影响。方法:24只健康清洁Wistar大鼠随机分为3组,每组8只。假手术组(S组)分离肠系膜上动脉(SMA),下腔静脉微量输液泵持续输注0.9%生理盐水10ml/(kg·h)。肠缺血再灌注组(I/R组)阻断SMA1h,下腔静脉微量输液泵持续输注0.9%生理盐水10ml/(kg·h)。异丙酚组(P组)阻断SMA1h,再灌注前5min将持续输注的0.9%生理盐水10ml/(kg·h)更换为等容异丙酚10mg/(kg·h)。于再灌注2h后下腔静脉取血,测定血尿素氮(BUN)和血肌酐(Bcr);处死大鼠后取双侧肾,右肾皮质部制作组织均浆测定超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,左肾作病理标本。结果:与S组相比,I/R组SOD活性降低,MDA、BUN及Bcr含量升高(P<0.05);与I/R组相比,P组MDA、BUN及Bcr含量降低,SOD活性升高(P<0.05)。病理结果显示:光镜下I/R组肾小球及球后毛细血管明显扩张,细胞水肿,球后毛细血管内堆积大量破裂红细胞;P组肾结构接近S组。结论:大鼠肠缺血再灌注后可造成肾损伤,异丙酚对其具有保护作用。
Objective： To investigate the effects of propofol on the kidney injury induced by intestinal ischemia/reperfusion （I/R） in rats and the underlying mechanism. Methods： Twenty-four Wistar rats of male weighing 280-330 g were randomly divided into 3 equal groups （n=8 each）. Superior mesenteric artery （SMA） were separated from abdominal cavity and continuously injected 0.9% Sodium Chloride 10 ml/（kg·h） by pump in sham operation group （S）. Intestinal ischemia/reperfusion was produced by clamping SMA for 1 h followed by 2 h reperfusion and continuously injected 0.9% Sodium Chloride 10 ml/（kg·h） by pump in I/R group. Propofol group （P） received propofol 10 mg/（kg· h） intravenously 5 min before reperfusion. We used instrument to determine BUN and Bcr from venous blood. Both kidneys were taken out of rats which were sacrificed by decapitation at the end. Cortical part of the right kidney was made into homogenate, measuring the determination of SOD activity and MDA content. The left was conserved by 10% Liquor Formaldehyde. The structural changes of kidney were examined by microscopy with HE staining. Results： The SOD activity was significantly lower while the MDA content was higher in I/R group than in S group （P〈0.05）. The SOD activity was significantly higher while the MDA content was lower in P group than in I/R group （P〈0.05）. Under the microscope, we found that glomcrulus and postglomerular capillary were expanded, cellularedema, ruptured erythrocyte were accumulated in postglomerular capillary. Conclusion： Intestinal ischemia/reperfusion can induce kidney injury, but propofol can suppress this injury.
出处
《天津医科大学学报》
2009年第4期683-685,共3页
Journal of Tianjin Medical University
关键词
异丙酚
缺血再灌注
肾
鼠
Propofol
Ischemia/reperfusion
Kidney
Rats
