摘要
目的探讨异丙酚对内毒素诱导的大鼠肺泡Ⅱ型上皮细胞Toll样受体4（TLR4）表达水平的影响。方法SPF级雄性Wistar大鼠，体重180～250g，8～9周龄，原代培养大鼠肺泡Ⅱ型上皮细胞，经鉴定后随机分为5组，每组18孔，对照组（C组）：不给予任何药物，继续培养3h；LPS组：加入LPS，终浓度1μg／ml，孵育3h；异丙酚组（P1-3组）：同时加入脯（终浓度1μg／ml）和终浓度分别为25、50、100μmol／L的异丙酚，孵育3h。孵育结束后测定肺泡Ⅱ型上皮细胞TLR4 mRNA、TLR4蛋白表达和肿瘤坏死因子α（TNF-α）的释放量。结果与C组比较，LPS组和P1组TLR4 mRNA及其蛋白表达上调（P〈0．05），P2组和P3组差异无统计学意义（P〉0．05）；与LPS组比较，P2组和琢组TLR4 mRNA和其蛋白表达下调，TNF—α释放量降低（P〈0．05或0．01），P1组上述指标差异无统计学意义（P〉0．05）；P2组与P1组上述指标差异无统计学意义（P〉0．05）。结论异丙酚可抑制LPS诱导的大鼠肺泡Ⅱ型上皮细胞TLR4 mRNA及其蛋白表达上调，且呈浓度依赖性，这可能是其抑制肺局部炎性反应的机制。
Objective To investigate the effect of propofol on LPS-induced TLR4 expression in rat alveolar type Ⅱ epithelial cells. Methods The primarily cultured alveolar type Ⅱepithelial cells isolated from male rats were randomly assigned to one of 5 groups： group Ⅰ cells were incubated for 3 h without any additive （control） ; group Ⅱ cells were incubated with LPS 1 μg/ml for 3 h （LPS） ; group Ⅲ , Ⅳ, Ⅴcells were incubated with LPS 1 μg/ml ＋ propofol 25, 50 and 100 μmol/L respectively for 3 h （P1-3） . TLR4 mRNA and TLRg protein expression was detected by real time PCR and Western blot. TNF-α release amount was measured using ELISA. Results LPS significantly increased TLR4 mRNA and protein expression in alveolar type Ⅱ epithelial cells as well as TNF-α release amount. Propofol at 50 and 100 μmol/L significantly inhibited LPS-induced increase in TLR4 mRNA and protein expression and TNF-α release amount. Conclusion Propofol can dose-dependently inhibit LPS- induced inflammation in alveolar type Ⅱepithelial cells, through down-regulation of TLR4 gene and protein expression.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2009年第8期749-752,共4页
Chinese Journal of Anesthesiology
关键词
二异丙酚
TOLL样受体4
上皮细胞
肺泡
肿瘤坏死因子Α
Propofol
Toll-like receptor 4
Epithelial cells
Pulmonary alveoli
Tumor necrosis factor-alpha
