摘要
目的:构建大鼠小肠缺血再灌注模型,观察谷氨酰胺强化肠外营养对小肠黏膜屏障作用的影响,并探讨其作用机制。方法:30只雌性Wistar大鼠,随机分为正常对照组(N组)、传统肠外营养组(TPN组)和谷氨酰胺强化肠外营养组(TPN+Gln组)3组,每组10只。TPN组和TPN+Gln组构建小肠缺血再灌注模型后予完全肠外营养5d。观察3组小肠黏膜形态、血浆D-乳酸、内毒素、TNF-α、IL-6水平及小肠黏膜HO-1 mRNA和蛋白的表达。结果:TPN+Gln组与TPN组相比,小肠黏膜组织形态明显改善,血浆D-乳酸、内毒素、TNF-α和IL-6水平均显著性降低,HO-1 mRNA及蛋白表达水平明显增高。结论:谷氨酰胺强化肠外营养可以明显减轻大鼠缺血再灌注小肠黏膜屏障损伤及炎性反应,保护黏膜屏障完整性,并促进HO-1 mRNA表达及HO-1合成。HO-1及其代谢产物的抗氧化、抗凋亡及抗炎作用可能是谷氨酰胺保护缺血再灌注损伤小肠的作用机制。
Objective：Small bowel IRI models in rats were established to explore the effect of glutamine enriched parenteral nutrition on mucosal barrier,and to discuss the probable mechanisms.Methods：Thirty Wistar rats were randomly assigned into 3 groups：The control group（N group,n=10） ,conducted fictitious operation and fed with common forage,TPN group（n=10） and TPN＋Glu group（n=10） .The morphous of mucous,serum and intestinal mucosal Gln concentration,levels of D-lactate,endotoxin,TNF-α,IL-6,HO-1 positive ratio and HO-1 mRNA were detected.Results：Glutamine obviously improved the structure of intestinal mucosal and decreased the expressions of D-lactate,endotoxin,TNF-αand IL-6.And enhanced the expressions of HO-1 mRNA and HO-1.Conclusion：Glutamine enriched parenteral nutrition can alleviate small intestinal IRI and inflammatory reaction and enhance the HO-1 and HO-1 mRNA expressions.HO-1 and its metabolin＇s anti-oxygen,anti-apoptosis,anti-inflammator action may be the mechanism of the protective action of Gln on mucosal barrier of small bowel.
出处
《中国现代普通外科进展》
CAS
2009年第10期837-841,共5页
Chinese Journal of Current Advances in General Surgery
