摘要
目的探讨StrepA快速诊断试剂在A族(乙型)溶血性链球菌(group A streptococcus,GAS)急性咽扁桃体炎、猩红热早期诊断中的价值。方法于2005年12月至2006年7月选取临床确诊猩红热患儿56例,记录临床资料及抗生素应用史,留取咽拭子同时进行细菌培养和快速抗原检测。于2006年11月至2007年5月选取临床疑似急性A族链球菌咽扁桃体炎患儿104例、病毒性上呼吸道感染患儿35例,记录临床资料及抗生素应用病史,进行A族链球菌链快速抗原检测。结果56例猩红热患儿咽拭子Quick Vue StrepA快速诊断试剂检测发现阳性51例(91.07%),细菌培养发现阳性30例(53.57%),两种检测方法有显著性差异(P<0.01)。30例细菌培养阳性标本,经Quick Vue StrepA快速诊断试剂检测阳性29例,符合率为96.67%;而51例经Quick Vue StrepA快速诊断试剂检测阳性标本,细菌培养阳性仅29例,符合率为56.86%。应用抗生素组细菌培养阳性率低于未应用抗生素组。104例临床疑似急性A链咽扁桃体患儿StrepA快速抗原检测阳性49例,阳性率为47.12%;35例诊断为病毒性上呼吸道感染患儿阳性1例,阳性率为2.86%。结论Quick Vue StrepA快速诊断试剂检测GAS急性咽扁桃体炎和猩红热敏感性高、特异性好,抗生素应用不影响快速抗原检测阳性率,适于临床快速病原学诊断。
Objective To investigate the value of StrepA rapid agents in the quick diagnosis of acute group A Streptococcus（GAS） pharyngealtonsillitis and scarlet fever. Methods The study was conducted at outpatient department of Beijing Children＇s Hospital from 2005 December to 2006 July. A total number of 56 patients with definite diagnosis of scarlet fever was enrolled. All clinical data including antibiotic usage were recorded. The throat swab specimens were collected for bacterial culture and rapid antigen detection. 104 patients diagnosed as suspected acute group A pharyngealtonsillitis and 35 patients diagnosed as viral upper respiratoy tract infections were enrolled. The group A rapid antigen detection was performed for all cases. Results Of 56 scarlet fever cases, 51 （91.07%） were confirmed by Quick Vue StrepA rapid diagnosis agent, and 30 （53.57%） were confirmed by bacterial culture only. The detection limit of Quick Vue StrepA rapid diagnosis agent and bacterial culture was significant （ P≤0.01 ）. 29 of 30 （96.67% ） patients with positive findings of both Quick Vue StrepA rapid diagnosis agent and bacterial culture. The positive finding of bacterial culture was lower in the group with previous antibiotic usage than those without antibiotic usage. 49 of 104 patients who were suspected to have acute GAS pharyngealtonsillitis were detected positive by Strep A rapid antigen agent, the positive rate was 47.12%. Whereas only 1 （2.86%） of 35 patients with suspected viral upper respiratory tract infection was positive. Conclusions Quick Vue StrepA rapid diagnose agent could be applied for rapid diagnosis of acute GAS pharyngealtonsillitis and scarlet fever in clinical setting for its high sensitive and specificity and not affected by previous antibiotic usage.
出处
《北京医学》
CAS
2009年第12期717-719,共3页
Beijing Medical Journal
