摘要
目的：建立苗格郎洗剂中苦参碱、氧化苦参碱含量的测定方法。方法：采用反相高效液相色谱法。色谱柱为ZORBAX NH2（5μm，4．6×150mm），流动相为乙腈-无水乙醇-3％磷酸溶液（80：10：10），柱温25℃，检测波长220nm。结果：苦参碱在0．253—1．264mg与峰面积积分值呈良好的线形关系（r=0．9999），平均加样回收率为98．13％，RSD为1．02％；氧化苦参碱在0．1424．0．9968μg与峰面积积分值呈良好的线形关系（r=0．9999），平均加样回收率为98．05％，RSD为0．88％。结论：采用反相高效液相色谱法测定苦参碱、氧化苦参碱的含量．方法简便、灵敏、准确．可用于苗格郎洗剂的质量控制。
Objective： To delvelop a method determining th content of Matrine and Oxymatrine in Miao Ge Long Lotion by RP - HPLC . Methods ： The analysis was carried on a column of ZORBAX NH2 （5μm, 4. 6mm × 150 mm） with a mobile phase of acetonitrile-ethanol- 3% phosphoric acid （80： 10： 10） was detected wavelength at 220nm with flowing rate as 1.0ml. min -1. Results ： The calibration curve of Matrine content was linear within range of 0. 253 - 1. 264 mg （ r = 0. 999 9 ）, the average recovery of Matrine was 98.13% , RSD was 1.02 % ; the calibration curve of Oxymtrine content was linear within the range of 0. 1424 - 0. 9968 mg （r = 0. 999 9） , the average recovery of Oxymtrine 98.05 % , RSD was 0. 88 %. Conclusion ： The method is simple, fast and accurate, it can increase the quality control index of Miao Ge Lang.
出处
《中国民族民间医药》
2009年第23期1-2,共2页
Chinese Journal of Ethnomedicine and Ethnopharmacy
