摘要
目的应用噬菌体随机十二肽库生物淘选具有刺激表皮细胞增殖作用的人成纤维细胞生长因子-7(hFGF-7)噬菌体模拟肽。方法以hFGF-7单克隆抗体为靶,进行4轮生物淘选噬菌体随机十二肽库;随机挑取单克隆噬菌体,应用ELISA方法检测单克隆噬菌体的结合力,对结合力较好的单克隆噬菌体提取噬菌体DNA,进行测序,并行序列分析。结果经过4轮生物淘选,特异性噬菌体模拟肽获得了富集,ELISA检测结果显示获得的一些噬菌体模拟肽具有较好的结合力,测序获得11个与促进细胞分裂、增殖或抑制细胞增殖有关的碱基序列。结论从噬菌体随机十二肽库中可淘选到与hFGF-7相关的噬菌体模拟肽,其中部分噬菌体模拟肽可能会促进表皮细胞增殖,期望将来可应用于促进创面愈合。
Objective To harvest hFGF-7 modeling peptides from a phage random twelve peptide library to promote epidermal cells proliferation.Methods A phage random twelve peptide library was screened 4 times by monoclonal anti-human FGF-7.ELISA staining examination was performed to choose monoclonal phages at random.Also,DNA extracted from phages with better binding-activity was sequenced and gene-compared.Results After 4 times of screening,abundant of specific phage modeling peptides were harvested.The results of ELISA showed a fine associativity of some phage modeling peptides.A total of 11 sequences were recognized which were related to promoting cell division and proliferation or inhibiting cell proliferation.Conclusion hFGF-7 related phage modeling peptides can be harvested from a phage random twelve peptide library,in which some phage modeling peptides may promote epidermis cells proliferation.
出处
《山东大学学报（医学版）》
CAS
北大核心
2009年第11期46-49,共4页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金资助课题(30670571
30772258)
国家重点基础研究发展计划(973计划)资助项目(2005CB522603)
山东省教育厅科研发展计划资助项目(J07WD03)
