摘要
目的:探讨人参皂甙Rb1、Rg1在肾缺血/再灌注血清诱导HK-2细胞凋亡中对Bcl-2、Bax表达的影响。方法:制备家兔肾缺血/再灌注血清(SIR)和对照组血清(SC)用于HK-2细胞培养,TUNEL法检测细胞凋亡。实验分组:对照组、缺血/再灌注组、Rb1干预组、Rg1干预组,培养24 h后免疫细胞化学法检测Bcl-2、Bax的表达。结果:与缺血/再灌注组比较,Rb1干预组和Rg1干预组Bax的表达明显下降(P<0.01),Bcl-2/Bax比值增大。结论:人参皂甙Rb1、Rg1对肾缺血/再灌注血清诱导HK-2细胞凋亡具有保护作用。
To explore effects of Ginsenosides（Rb1, Rg1） on the expression of Bcl-2, Bax in the serum of kidney ischemia/reperfusion indu-cing apoptosis of HK-2 cells. Methods： The serum of rabbits with renal ischemia/reperfusion（SIR） and the control sertan of rabbits（SC） were acquired and cultured with HK-2 cells. Detected apoptosis with TUNEL assay. The experiment was designed as： control group, ischemia/ reperfusion group, Rb1 blocking group and Rg1 blocking group. To detect the expression of Bcl-2, Bax with immunocytochemistry after 24 hours＇ cultured. Results： The expression of Bax in Rb1 blocking group and Rg1 blocking group were significantly decreased （ P 〈 0.01）, the ratios of Bcl-2/Bax were increased as compared with ischemia/reperfusion group. Conclusion： Rb1 and Rg1 have protective effects on apoptosis of HK-2 cells induced by serum of kidney ischemia/reperfusion.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2009年第4期496-499,I0013,共5页
Chinese Journal of Applied Physiology
