摘要
目的:研究过氧化氢诱导NRK52E细胞凋亡模型的制作方法,探讨非促分裂型人酸性成纤维细胞生长因子(non-mitogenic human acidic fibroblast growth factor,nmhaFGF)对过氧化氢诱导的大鼠肾小管上皮细胞(NRK52E细胞)凋亡是否具有拮抗作用。方法:用MTT、Hoechst33342染色以及流式细胞仪检测确定诱导细胞凋亡的过氧化氢的浓度,制作NRK52E细胞凋亡模型,以不同浓度的nmhaFGF作用于凋亡细胞,并以haFGF作为对照,观察nmhaFGF对NRK52E细胞凋亡的影响。结果:0.4mmol/L过氧化氢作用NRK52E细胞18h是诱导NRK52E细胞凋亡的最佳条件。nmhaFGF0.01、0.03、0.10、0.30、1.00mg/L剂量范围,随着nmhaFGF浓度升高,细胞凋亡率明显降低,而相同浓度haFGF各剂量组细胞凋亡率呈现不同程度的降低,但haFGF剂量组量效关系不明显。结论:切除促分裂序列的haFGF可以发挥对NRK52E细胞凋亡的保护作用,该保护作用可能是通过其非促分裂活性实现的。
AIM： The present study was designed to establish H2O2 -induced NRK52E cell apoptotic model and to investigate the effects and the mechanism of nmhaFGF on the NRK52E cell apoptosis induced by H2O2. METHODS： In vitro experiment, a apoptotic model of normal rat kidney epithelium （NRK52E） was made hy MTT method, Hoechst33342 dyeing and flow cytosorting （FCR）. NRK52E cells were cultured with different concentrations of nmhaFGF and haFGF for 24 h before H2O2 was added. The apoptotic rate was detected with FCR method. RESULTS： H2O2 at concentration of 0.4 mmol/L, the optimal condition to establish the apoptotic model, was used to treat NRK52E cells for 18h. Different doses of nmhaFGF （0. 01, 0. 03, 0. 10, 0. 30, 1.00 mg/L） reduced the apoptotic rates with the dose rising. However, the decreasing tends of apoptotic rates with dose increasing for haFGF was not so obvious. CONCLUSION： nmhaFGF protects the NRK52E ceils against apoptosis. The mechanism might be connected with its non - mitogenic property.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2008年第11期2230-2234,共5页
Chinese Journal of Pathophysiology
基金
国家863计划资助项目(No.2002AA2Z3318
No.2004AA2Z3C60)
新乡医学院高学历人才资助项目
