摘要
目的:建立以高效液相色谱(HPLC)法测定龙胆泻肝丸中龙胆苦苷含量的方法。方法:色谱柱为VP-ODS(150mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(15∶85),检测波长为270nm,流速为1.0mL·min-1,柱温为25℃。结果:龙胆苦苷进样量在0.3554～5.331μg范围内与峰面积积分值呈良好线性关系(r=0.9998);平均回收率为100.05%,RSD=2.2%(n=6)。结论:本方法简便、快速、准确,可用于龙胆泻肝丸的质量控制。
OBJECTIVE： To establish an HPLC method for determination of gentiopicroside in Gentian liver - purging pill. METHODS： The chromatographic separation was carried out on VP ODS（150 mm× 4.6 mm, 5μm）. The mobile phase consisted of acetonitrile- 0.1% phosphoric acid solution（15 ： 85） at a flow rate of 1.0 mL·min^-1. The UV detection wavele ngth was set at 270 nm and column temperature was kept at 25 ℃ .RESULTS： The liner range of gentiopicroside was 0. 355 4- 5. 331 μg（r = 0.999 8） and the average recovery was 100.05% （RSD = 2.2%, n = 6）. CONCLUSION ： The method is simple, rapid and accurate, and it is applicable for the quality control of Gentian liver-purging pill.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第36期2857-2858,共2页
China Pharmacy
