摘要
本文建立了龙胆类生药中五种苦甙类成分,即:龙胆苦甙(gentiopicroside),当药苦甙(swertiamarin)、当药甙(sweroside)、苦龙胆酯甙(amarogentin)和苦当药酯甙(amaroswerin)的高效液相色谱(HPLC)定量分析方法,并对国产九种龙胆中这五种成分的含量进行了测定和比较。
A new and rapid analytical method for the simultaneous determination of five bitter secoiridoid glycosides( gentiopicroside, GTP; swertiamarin, SWT; sweroside, SWO; amarogentin, AMG; amaroswerin, AMS)in the Chinese drug 'Long Dan', roots of Gentiana manshurica and 8 allied species by high performance liquid chromatography (HPLC)has been developed. The HPLC system consisting of an apparatus from Shimadzu (model LC-4A), with a UV-detector(SPD-2AS), a data processor (Chromatopac C-R2AX), a column oven (CTO- 2AS), and a Zorbax ODS column (25 cm×4.6 mm ID) was used. Using MeOH -- H_2O as the mobile phase, the linear concentration-program of methanol was [time (min) : C (MeOH)] : [0:20]→[6 : 20]→[ 6.01: 401→[23:stop]. The temperature of column oven was 40℃. The bitter secoiridoid glycosides eluted were detected at a wavelength of 254 nm and the analysis was successfully carried out within 23 minutes. This method is sensitive, rapid, accurate and has good reproducibility. Recoveries of each secoiridoid glycoside were 100.0～101.5% with coefficients of variation 0～2.5% (n=3). The contents of five bitter secoiridoid glycosides in the roots of G. manshurica and eight allied species indigenous to China were determined and reported.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第11期864-870,共7页
Acta Pharmaceutica Sinica
关键词
龙胆
当药甙
龙胆苦甙
HPLC
Radix Gentianae (Long Dan)
Gentiopicroside
Swertiamarin
Sweroside
Amarogentin
Amaroswerin
