摘要
菲律宾蛤仔软体部经匀浆、乙醇提取、旋转蒸发浓缩,得到菲律宾蛤仔乙醇提取物,乙醇提取物经有机溶剂萃取,依次得到乙酸乙酯萃取物、石油醚萃取物和水溶性组分。在32℃,pH=6.8的Na2HPO4-NaH2PO4缓冲体系中,研究菲律宾蛤仔提取物对酪氨酸酶活力的抑制作用。结果表明,菲律宾蛤仔乙醇提取物对酪氨酸酶有明显的抑制作用,其IC50为0.388 mg/mL。进一步动力学分析表明,乙酸乙酯萃取物、石油醚萃取物对酪氨酸酶具有非竞争性可逆抑制作用,水溶性组分对酪氨酸酶具有竞争性可逆抑制作用。
The soft tissue of Ruditapes philippinarum was separated to obtain the extract by pounding to pieces, marinating in ethanol and distilling to a concentrated liquid. The inhibitory capability of the extract from Ruditapes philippinarum against tyrosinase activity was studied with Na2HPO4-NaH2PO4 as buffer solution（pH = 6.8） and at 32 ℃. The results showed that the ethanolic extract from Ruditapes philippinarurn can inhibit the activitiy of tyrosinase obviously, the concentration corresponding to 50% inhibitory rate（ICs0） is 0.388 mg/mL. The ethanolic extract was leached with ethyl acetate and petroleum ether. Lineweaver-Burk plots demonstrate that the inhibition effect of the ethyl acetate-soluble fraction and the petroleum ether-soluble fraction express as what a noncompetitive inhibitor does, the substance in water expresses as what a competitive inhibitor does.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第6期1233-1236,1260,共5页
Periodical of Ocean University of China
基金
国家自然科学基金项目(30672515)资助
