摘要
对小鼠分别灌胃200、400、800 mg/(kg.d)的海带岩藻聚糖硫酸酯(简称FLJ,利用酶解法制备),于第7天腹腔注射四氯化碳制备小鼠急性肝损伤模型,以200 mg/(kg.d)的联苯双酯做阳性对照。测定血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性及肝组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性,并观察肝组织形态学变化,研究FLJ对CCl4诱导急性肝损伤小鼠的保护作用。结果表明:FLJ各剂量均能抑制肝损伤小鼠血清ALT、AST活性的升高,且中、高剂量组达到显著水平,并能显著提高肝组织中SOD活力、降低MDA含量,减轻由对肝细胞的病理损伤。FLJ对CCl4造成的小鼠急性肝损伤具有一定的保护作用。
Fucoidan was prepared from Laminaria japonica by enzymatic hydrolysis. Mice were pretreated with fucoidan from Laminaria japonica （200,400,800 mg/kg respectively for 7 days）. Then liver injury model was established by administration of CCl4 in mice on the 7th day. The positive control was administrated with 200 mg/kg dimethyl diphenyl bicarboxylate. The activities of serum ALT and AST, the content of MDA and the activity of SOD in hepatic tissue were measured. The hepatic histological changes were observed by optical microscope. The results showed that fucoidan from Larninaria japonica had significantly hepatoprotective activity on CCl4 induced liver damage in mice. Mice treated with fueoidan remarkably prevented the elevation of serum AST, ALT and liver lipid peroxides in CCl4 treated mice（P〈0. 01）. Histopathological changes induced by CCl4 were also significantly reduced by fucoidan treatment.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2009年第6期764-766,共3页
Journal of Huazhong Agricultural University
基金
国家农业部"948"项目(2006G44)资助
关键词
海带
岩藻聚糖硫酸酯
四氯化碳
肝保护
Laminaria japonica
fucoidan from Laminaria japonica
carbon tetrachloride
hepatoprotective
