摘要
介绍了一种红树ＲＮＡ提取方法，此法无需特殊试剂和贵重仪器设备，可有效地排除红树细胞中大量存在的胶质和多糖类物质的干扰，所得ＲＮＡ样品经紫外分光光度计检测和１％琼脂糖凝胶电泳分析证明具有较高的纯度和完整性，可满足多数分子生物学实验的需要，尤其适用于提取多糖含量较高的植物细胞的ＲＮＡ．
A simple extraction method for the total RNA of Rhizophora apiculata is introduced here.This method requires no special reagent and valuable instrument.It can efficiently eliminate the interferene of the polysaccharide which is very rich in Rhizophora apiculata. The results of 1% agarose gel electrophoresis and ultraviolet spectrophotometer analysis show that the total RNA of Rhizophora apiculata extracted through this method has no obvious degradation and has a good purity and can meet the needs of most molecular biological experiments.It is especially useful for the RNA extraction of plant cell which usually has plenty of polysaccharide.
出处
《海南大学学报（自然科学版）》
CAS
1998年第4期311-313,共3页
Natural Science Journal of Hainan University
基金
国家计委"九五"重点科技攻关项目
