摘要
目的对胃癌、胃淋巴瘤中EB病毒(EBV)的感染情况进行检测和分析,探讨EBV感染可能与胃肿瘤的病因关系。方法采用原位分子杂交(ISH)技术检测109例胃癌和30例胃淋巴瘤组织中EBV编码的小RNA(EBER),确定EBV在胃癌和胃淋巴瘤细胞中的存在。结果EBER阳性存在于胃癌细胞核和胃淋巴瘤细胞核内。胃癌组织中EBER阳性率为11.0%(12例/109例),胃淋巴瘤组织中EBER阳性率为30.0%(9例/30例)。胃淋巴瘤EBV阳性率高于胃癌(P=0.022)。但EB病毒感染在病人不同年龄、性别、肿瘤部位、组织学类型、临床分期和淋巴结转移中的差异没有统计学意义(P>0.05)。结论EB病毒感染可能参与某些胃癌和胃淋巴瘤的发生与进展。
Objective Epstein - Barr virus （EBV） may associate with the genesis and progress of gastric carcinoma. The study was to explore the correlation between infection of EBV and gastric carcinoma and gastric lymphoma. Methods EBER was detected in 109 specimens of gastric carcinoma paraffin - embedded tissues and in 30 specimens of gastric lymphoma paraffin - embedded tissues by in situ hybridization （ ISH ）. Results EBER was expressed in tumor cell nucleus. The rates of positive EBER was 11.0% （ 12/109 ） and 30.0% （ 9/30 ） from gastric carcinoma and gastric lymphoma, respectively. The difference between the groups was of statistical significance （ P = 0.022 ）. Lymph node metastasis - rate in EBV positive gastric carcinoma （ EBVaGC ） was lower than that in EBV negative gastric carcinoma （ EBVnGC, P = 0.033 ）. In contrast, there was no statistically significant difference between EBV positive gastric carcinoma and EBV negative gastric carcinoma with regard to age, sex, site, histological type, clinical stage （ early or advanced ） or lymph node metastasis （P 〉 0. 05 ）. Conclusions The results suggest that EBV infection may partially contribute to the genesis and progress of gastric carcinoma and gastric lymphoma.
出处
《南华大学学报（医学版）》
2009年第6期658-661,共4页
Journal of Nanhua University(Medical Edition)
基金
国家自然科学基金(30772116)
湖南省自然科学基金(08JJ3034)
南华大学博士启动基金(2007XQD25)
关键词
胃肿瘤
腺癌
淋巴瘤
EB病毒
EBER
gastric tumor
adenocarcinoma
lymphoma
Epstein-Barr virus
EBER
